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Dual-barcoded shotgun expression library sequencing for high-throughput characterization of functional traits in bacteria
A major challenge in genomics is the knowledge gap between sequence and its encoded function. Gain-of-function methods based on gene overexpression are attractive avenues for phenotype-based functional screens, but are not easily applied in high-throughput across many experimental conditions. Here,...
Autores principales: | Mutalik, Vivek K., Novichkov, Pavel S., Price, Morgan N., Owens, Trenton K., Callaghan, Mark, Carim, Sean, Deutschbauer, Adam M., Arkin, Adam P. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6338753/ https://www.ncbi.nlm.nih.gov/pubmed/30659179 http://dx.doi.org/10.1038/s41467-018-08177-8 |
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