Activation of silent biosynthetic gene clusters using transcription factor decoys

Here we report a transcription factor decoy strategy for targeted activation of eight large silent polyketide synthase and non-ribosomal peptide synthetase gene clusters, ranging from 50 to 134 kilobases in multiple streptomycetes, and characterization of a novel oxazole family compound produced by...

Descripción completa

Detalles Bibliográficos
Autores principales: Wang, Bin, Guo, Fang, Dong, Shi-Hui, Zhao, Huimin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6339570/
https://www.ncbi.nlm.nih.gov/pubmed/30598544
http://dx.doi.org/10.1038/s41589-018-0187-0
_version_ 1783388657191747584
author Wang, Bin
Guo, Fang
Dong, Shi-Hui
Zhao, Huimin
author_facet Wang, Bin
Guo, Fang
Dong, Shi-Hui
Zhao, Huimin
author_sort Wang, Bin
collection PubMed
description Here we report a transcription factor decoy strategy for targeted activation of eight large silent polyketide synthase and non-ribosomal peptide synthetase gene clusters, ranging from 50 to 134 kilobases in multiple streptomycetes, and characterization of a novel oxazole family compound produced by a 98-kb biosynthetic gene cluster. Due to its simplicity and ease, this strategy can be readily scaled up for discovery of natural products in streptomycetes.
format Online
Article
Text
id pubmed-6339570
institution National Center for Biotechnology Information
language English
publishDate 2018
record_format MEDLINE/PubMed
spelling pubmed-63395702019-06-30 Activation of silent biosynthetic gene clusters using transcription factor decoys Wang, Bin Guo, Fang Dong, Shi-Hui Zhao, Huimin Nat Chem Biol Article Here we report a transcription factor decoy strategy for targeted activation of eight large silent polyketide synthase and non-ribosomal peptide synthetase gene clusters, ranging from 50 to 134 kilobases in multiple streptomycetes, and characterization of a novel oxazole family compound produced by a 98-kb biosynthetic gene cluster. Due to its simplicity and ease, this strategy can be readily scaled up for discovery of natural products in streptomycetes. 2018-12-31 2019-02 /pmc/articles/PMC6339570/ /pubmed/30598544 http://dx.doi.org/10.1038/s41589-018-0187-0 Text en Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Wang, Bin
Guo, Fang
Dong, Shi-Hui
Zhao, Huimin
Activation of silent biosynthetic gene clusters using transcription factor decoys
title Activation of silent biosynthetic gene clusters using transcription factor decoys
title_full Activation of silent biosynthetic gene clusters using transcription factor decoys
title_fullStr Activation of silent biosynthetic gene clusters using transcription factor decoys
title_full_unstemmed Activation of silent biosynthetic gene clusters using transcription factor decoys
title_short Activation of silent biosynthetic gene clusters using transcription factor decoys
title_sort activation of silent biosynthetic gene clusters using transcription factor decoys
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6339570/
https://www.ncbi.nlm.nih.gov/pubmed/30598544
http://dx.doi.org/10.1038/s41589-018-0187-0
work_keys_str_mv AT wangbin activationofsilentbiosyntheticgeneclustersusingtranscriptionfactordecoys
AT guofang activationofsilentbiosyntheticgeneclustersusingtranscriptionfactordecoys
AT dongshihui activationofsilentbiosyntheticgeneclustersusingtranscriptionfactordecoys
AT zhaohuimin activationofsilentbiosyntheticgeneclustersusingtranscriptionfactordecoys

Ejemplares similares