A Bacterial Expression Vector Archive (BEVA) for Flexible Modular Assembly of Golden Gate-Compatible Vectors

We present a Bacterial Expression Vector Archive (BEVA) for the modular assembly of bacterial vectors compatible with both traditional and Golden Gate cloning, utilizing the Type IIS restriction enzyme Esp3I, and have demonstrated its use for Golden Gate cloning in Escherichia coli. Ideal for synthe...

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Detalles Bibliográficos
Autores principales: Geddes, Barney A., Mendoza-Suárez, Marcela A., Poole, Philip S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2019
Materias:
Microbiology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6339899/
https://www.ncbi.nlm.nih.gov/pubmed/30692983
http://dx.doi.org/10.3389/fmicb.2018.03345

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6339899/
https://www.ncbi.nlm.nih.gov/pubmed/30692983
http://dx.doi.org/10.3389/fmicb.2018.03345

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