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In vitro antihypertensive and antioxidative properties of trypsin‐derived Moringa oleifera seed globulin hydrolyzate and its membrane fractions

Moringa oleifera seed globulin was hydrolyzed with trypsin and fractionated to produce <1, 1–3, and 3–5 kDa peptide sizes. These were evaluated for antioxidant properties: DPPH, hydroxyl radical scavenging assays, FRAP, and metal chelation tests; and in vitro antihypertensive properties: ACE and...

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Autores principales: Aderinola, Taiwo Ayodele, Fagbemi, Tayo Nathaniel, Enujiugha, Victor Ndigwe, Alashi, Adeola Monisola, Aluko, Rotimi Emmanuel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6341156/
https://www.ncbi.nlm.nih.gov/pubmed/30680166
http://dx.doi.org/10.1002/fsn3.826
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author Aderinola, Taiwo Ayodele
Fagbemi, Tayo Nathaniel
Enujiugha, Victor Ndigwe
Alashi, Adeola Monisola
Aluko, Rotimi Emmanuel
author_facet Aderinola, Taiwo Ayodele
Fagbemi, Tayo Nathaniel
Enujiugha, Victor Ndigwe
Alashi, Adeola Monisola
Aluko, Rotimi Emmanuel
author_sort Aderinola, Taiwo Ayodele
collection PubMed
description Moringa oleifera seed globulin was hydrolyzed with trypsin and fractionated to produce <1, 1–3, and 3–5 kDa peptide sizes. These were evaluated for antioxidant properties: DPPH, hydroxyl radical scavenging assays, FRAP, and metal chelation tests; and in vitro antihypertensive properties: ACE and renin inhibition. Membrane fractionation led to improved antioxidative properties of 29.13% (<1 kDa), 180% (<1 kDa), and 30.58% (1–3 kDa) for DPPH, FRAP, and metal iron chelation, respectively. There was however 48.77% reduction (1–3 kDa) in hydroxyl radical scavenging activity. There was also improvement in ACE inhibitory potentials of the peptides with the 1–3 kDa peptide showing significantly highest ACE inhibition (72.48%)but very low (17.64%, 1–3 kDa) inhibition against the renin. It was concluded that hydrolysis of M oleifera seed globulin with trypsin produced peptides and peptide fractions with potential antioxidant and antihypertensive properties.
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spelling pubmed-63411562019-01-24 In vitro antihypertensive and antioxidative properties of trypsin‐derived Moringa oleifera seed globulin hydrolyzate and its membrane fractions Aderinola, Taiwo Ayodele Fagbemi, Tayo Nathaniel Enujiugha, Victor Ndigwe Alashi, Adeola Monisola Aluko, Rotimi Emmanuel Food Sci Nutr Original Research Moringa oleifera seed globulin was hydrolyzed with trypsin and fractionated to produce <1, 1–3, and 3–5 kDa peptide sizes. These were evaluated for antioxidant properties: DPPH, hydroxyl radical scavenging assays, FRAP, and metal chelation tests; and in vitro antihypertensive properties: ACE and renin inhibition. Membrane fractionation led to improved antioxidative properties of 29.13% (<1 kDa), 180% (<1 kDa), and 30.58% (1–3 kDa) for DPPH, FRAP, and metal iron chelation, respectively. There was however 48.77% reduction (1–3 kDa) in hydroxyl radical scavenging activity. There was also improvement in ACE inhibitory potentials of the peptides with the 1–3 kDa peptide showing significantly highest ACE inhibition (72.48%)but very low (17.64%, 1–3 kDa) inhibition against the renin. It was concluded that hydrolysis of M oleifera seed globulin with trypsin produced peptides and peptide fractions with potential antioxidant and antihypertensive properties. John Wiley and Sons Inc. 2018-11-25 /pmc/articles/PMC6341156/ /pubmed/30680166 http://dx.doi.org/10.1002/fsn3.826 Text en © 2018 The Authors. Food Science & Nutrition published by Wiley Periodicals, Inc. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Research
Aderinola, Taiwo Ayodele
Fagbemi, Tayo Nathaniel
Enujiugha, Victor Ndigwe
Alashi, Adeola Monisola
Aluko, Rotimi Emmanuel
In vitro antihypertensive and antioxidative properties of trypsin‐derived Moringa oleifera seed globulin hydrolyzate and its membrane fractions
title In vitro antihypertensive and antioxidative properties of trypsin‐derived Moringa oleifera seed globulin hydrolyzate and its membrane fractions
title_full In vitro antihypertensive and antioxidative properties of trypsin‐derived Moringa oleifera seed globulin hydrolyzate and its membrane fractions
title_fullStr In vitro antihypertensive and antioxidative properties of trypsin‐derived Moringa oleifera seed globulin hydrolyzate and its membrane fractions
title_full_unstemmed In vitro antihypertensive and antioxidative properties of trypsin‐derived Moringa oleifera seed globulin hydrolyzate and its membrane fractions
title_short In vitro antihypertensive and antioxidative properties of trypsin‐derived Moringa oleifera seed globulin hydrolyzate and its membrane fractions
title_sort in vitro antihypertensive and antioxidative properties of trypsin‐derived moringa oleifera seed globulin hydrolyzate and its membrane fractions
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6341156/
https://www.ncbi.nlm.nih.gov/pubmed/30680166
http://dx.doi.org/10.1002/fsn3.826
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