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Streamlined procedure for gene knockouts using all-in-one adenoviral CRISPR-Cas9
CRISPR-Cas9 is a powerful gene editing technique that can induce mutations in a target gene of interest in almost any mammalian cell line. However, its practicality can be limited if target cell lines are difficult to transfect and do not proliferate. In the current study, we have developed a stream...
| Autores principales: | , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Nature Publishing Group UK
2019
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6342919/ https://www.ncbi.nlm.nih.gov/pubmed/30670765 http://dx.doi.org/10.1038/s41598-018-36736-y |