Degradation of endogenous proteins and generation of a null-like phenotype in zebrafish using Trim-Away technology

Trim-Away is a recent technique to rapidly deplete a protein from any cell type. Guided by antibodies, TRIM21 selects proteins for destruction. However, the applicability of this method in model organisms has not been investigated. Here, we show that Trim-Away can degrade proteins in zebrafish embry...

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Autores principales: Chen, Xiao, Liu, Mi, Lou, Hongyan, Lu, Yiyi, Zhou, Meng-Tao, Ou, Rongying, Xu, Yunsheng, Tang, Kai-Fu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2019
Materias:
Short Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6343325/
https://www.ncbi.nlm.nih.gov/pubmed/30674345
http://dx.doi.org/10.1186/s13059-019-1624-4
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author Chen, Xiao
Liu, Mi
Lou, Hongyan
Lu, Yiyi
Zhou, Meng-Tao
Ou, Rongying
Xu, Yunsheng
Tang, Kai-Fu
author_facet Chen, Xiao
Liu, Mi
Lou, Hongyan
Lu, Yiyi
Zhou, Meng-Tao
Ou, Rongying
Xu, Yunsheng
Tang, Kai-Fu
author_sort Chen, Xiao
collection PubMed
description Trim-Away is a recent technique to rapidly deplete a protein from any cell type. Guided by antibodies, TRIM21 selects proteins for destruction. However, the applicability of this method in model organisms has not been investigated. Here, we show that Trim-Away can degrade proteins in zebrafish embryos. Trim-Away depletes proteins faster than morpholinos, which enables analysis of protein function during early embryogenesis. Furthermore, Trim-Away can be applied to evaluate the role of maternally contributed proteins in zebrafish embryos. Our findings indicate that Trim-Away is a powerful tool to perform functional analysis of proteins during zebrafish development. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s13059-019-1624-4) contains supplementary material, which is available to authorized users.
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spelling pubmed-63433252019-01-24 Degradation of endogenous proteins and generation of a null-like phenotype in zebrafish using Trim-Away technology Chen, Xiao Liu, Mi Lou, Hongyan Lu, Yiyi Zhou, Meng-Tao Ou, Rongying Xu, Yunsheng Tang, Kai-Fu Genome Biol Short Report Trim-Away is a recent technique to rapidly deplete a protein from any cell type. Guided by antibodies, TRIM21 selects proteins for destruction. However, the applicability of this method in model organisms has not been investigated. Here, we show that Trim-Away can degrade proteins in zebrafish embryos. Trim-Away depletes proteins faster than morpholinos, which enables analysis of protein function during early embryogenesis. Furthermore, Trim-Away can be applied to evaluate the role of maternally contributed proteins in zebrafish embryos. Our findings indicate that Trim-Away is a powerful tool to perform functional analysis of proteins during zebrafish development. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s13059-019-1624-4) contains supplementary material, which is available to authorized users. BioMed Central 2019-01-23 /pmc/articles/PMC6343325/ /pubmed/30674345 http://dx.doi.org/10.1186/s13059-019-1624-4 Text en © The Author(s). 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Short Report
Chen, Xiao
Liu, Mi
Lou, Hongyan
Lu, Yiyi
Zhou, Meng-Tao
Ou, Rongying
Xu, Yunsheng
Tang, Kai-Fu
Degradation of endogenous proteins and generation of a null-like phenotype in zebrafish using Trim-Away technology
title Degradation of endogenous proteins and generation of a null-like phenotype in zebrafish using Trim-Away technology
title_full Degradation of endogenous proteins and generation of a null-like phenotype in zebrafish using Trim-Away technology
title_fullStr Degradation of endogenous proteins and generation of a null-like phenotype in zebrafish using Trim-Away technology
title_full_unstemmed Degradation of endogenous proteins and generation of a null-like phenotype in zebrafish using Trim-Away technology
title_short Degradation of endogenous proteins and generation of a null-like phenotype in zebrafish using Trim-Away technology
title_sort degradation of endogenous proteins and generation of a null-like phenotype in zebrafish using trim-away technology
topic Short Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6343325/
https://www.ncbi.nlm.nih.gov/pubmed/30674345
http://dx.doi.org/10.1186/s13059-019-1624-4
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