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Mutant T4 DNA polymerase for easy cloning and mutagenesis
The advent of high-fidelity DNA polymerases that can be used to linearize and amplify whole plasmids by PCR opened the door to greatly simplified cloning and mutagenesis protocols. Commercially available kits work well, but often have been optimized using undisclosed or proprietory components. Here...
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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Public Library of Science
2019
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6343910/ https://www.ncbi.nlm.nih.gov/pubmed/30673756 http://dx.doi.org/10.1371/journal.pone.0211065 |