Assessing baloxavir susceptibility of influenza viruses circulating in the United States during the 2016/17 and 2017/18 seasons

The anti-influenza therapeutic baloxavir targets cap-dependent endonuclease activity of polymerase acidic (PA) protein. We monitored baloxavir susceptibility in the United States with next generation sequencing analysis supplemented by phenotypic one-cycle infection assay. Analysis of PA sequences o...

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Autores principales: Gubareva, Larisa V, Mishin, Vasiliy P, Patel, Mira C, Chesnokov, Anton, Nguyen, Ha T, De La Cruz, Juan, Spencer, Sarah, Campbell, Angela P, Sinner, Mallory, Reid, Heather, Garten, Rebecca, Katz, Jackie M, Fry, Alicia M, Barnes, John, Wentworth, David E
Formato: Online Artículo Texto
Lenguaje:English
Publicado: European Centre for Disease Prevention and Control (ECDC) 2019
Materias:
Rapid Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6344838/
https://www.ncbi.nlm.nih.gov/pubmed/30670144
http://dx.doi.org/10.2807/1560-7917.ES.2019.24.3.1800666
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author Gubareva, Larisa V
Mishin, Vasiliy P
Patel, Mira C
Chesnokov, Anton
Nguyen, Ha T
De La Cruz, Juan
Spencer, Sarah
Campbell, Angela P
Sinner, Mallory
Reid, Heather
Garten, Rebecca
Katz, Jackie M
Fry, Alicia M
Barnes, John
Wentworth, David E
author_facet Gubareva, Larisa V
Mishin, Vasiliy P
Patel, Mira C
Chesnokov, Anton
Nguyen, Ha T
De La Cruz, Juan
Spencer, Sarah
Campbell, Angela P
Sinner, Mallory
Reid, Heather
Garten, Rebecca
Katz, Jackie M
Fry, Alicia M
Barnes, John
Wentworth, David E
author_sort Gubareva, Larisa V
collection PubMed
description The anti-influenza therapeutic baloxavir targets cap-dependent endonuclease activity of polymerase acidic (PA) protein. We monitored baloxavir susceptibility in the United States with next generation sequencing analysis supplemented by phenotypic one-cycle infection assay. Analysis of PA sequences of 6,891 influenza A and B viruses collected during 2016/17 and 2017/18 seasons showed amino acid substitutions: I38L (two A(H1N1)pdm09 viruses), E23G (two A(H1N1)pdm09 viruses) and I38M (one A(H3N2) virus); conferring 4–10-fold reduced susceptibility to baloxavir.
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spelling pubmed-63448382019-02-11 Assessing baloxavir susceptibility of influenza viruses circulating in the United States during the 2016/17 and 2017/18 seasons Gubareva, Larisa V Mishin, Vasiliy P Patel, Mira C Chesnokov, Anton Nguyen, Ha T De La Cruz, Juan Spencer, Sarah Campbell, Angela P Sinner, Mallory Reid, Heather Garten, Rebecca Katz, Jackie M Fry, Alicia M Barnes, John Wentworth, David E Euro Surveill Rapid Communication The anti-influenza therapeutic baloxavir targets cap-dependent endonuclease activity of polymerase acidic (PA) protein. We monitored baloxavir susceptibility in the United States with next generation sequencing analysis supplemented by phenotypic one-cycle infection assay. Analysis of PA sequences of 6,891 influenza A and B viruses collected during 2016/17 and 2017/18 seasons showed amino acid substitutions: I38L (two A(H1N1)pdm09 viruses), E23G (two A(H1N1)pdm09 viruses) and I38M (one A(H3N2) virus); conferring 4–10-fold reduced susceptibility to baloxavir. European Centre for Disease Prevention and Control (ECDC) 2019-01-17 /pmc/articles/PMC6344838/ /pubmed/30670144 http://dx.doi.org/10.2807/1560-7917.ES.2019.24.3.1800666 Text en This article is copyright of the authors or their affiliated institutions, 2019. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution (CC BY 4.0) Licence. You may share and adapt the material, but must give appropriate credit to the source, provide a link to the licence, and indicate if changes were made.
spellingShingle Rapid Communication
Gubareva, Larisa V
Mishin, Vasiliy P
Patel, Mira C
Chesnokov, Anton
Nguyen, Ha T
De La Cruz, Juan
Spencer, Sarah
Campbell, Angela P
Sinner, Mallory
Reid, Heather
Garten, Rebecca
Katz, Jackie M
Fry, Alicia M
Barnes, John
Wentworth, David E
Assessing baloxavir susceptibility of influenza viruses circulating in the United States during the 2016/17 and 2017/18 seasons
title Assessing baloxavir susceptibility of influenza viruses circulating in the United States during the 2016/17 and 2017/18 seasons
title_full Assessing baloxavir susceptibility of influenza viruses circulating in the United States during the 2016/17 and 2017/18 seasons
title_fullStr Assessing baloxavir susceptibility of influenza viruses circulating in the United States during the 2016/17 and 2017/18 seasons
title_full_unstemmed Assessing baloxavir susceptibility of influenza viruses circulating in the United States during the 2016/17 and 2017/18 seasons
title_short Assessing baloxavir susceptibility of influenza viruses circulating in the United States during the 2016/17 and 2017/18 seasons
title_sort assessing baloxavir susceptibility of influenza viruses circulating in the united states during the 2016/17 and 2017/18 seasons
topic Rapid Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6344838/
https://www.ncbi.nlm.nih.gov/pubmed/30670144
http://dx.doi.org/10.2807/1560-7917.ES.2019.24.3.1800666
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