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Insights From Deep Sequencing of the HBV Genome—Unique, Tiny, and Misunderstood

Hepatitis B virus (HBV) is a unique, tiny, partially double-stranded, reverse-transcribing DNA virus with proteins encoded by multiple overlapping reading frames. The substitution rate is surprisingly high for a DNA virus, but lower than that of other reverse transcribing organisms. More than 260 mi...

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Autores principales: McNaughton, Anna L., D’Arienzo, Valentina, Ansari, M. Azim, Lumley, Sheila F., Littlejohn, Margaret, Revill, Peter, McKeating, Jane A., Matthews, Philippa C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: by the AGA Institute. Published by Elsevier Inc. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6347571/
https://www.ncbi.nlm.nih.gov/pubmed/30268787
http://dx.doi.org/10.1053/j.gastro.2018.07.058
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author McNaughton, Anna L.
D’Arienzo, Valentina
Ansari, M. Azim
Lumley, Sheila F.
Littlejohn, Margaret
Revill, Peter
McKeating, Jane A.
Matthews, Philippa C.
author_facet McNaughton, Anna L.
D’Arienzo, Valentina
Ansari, M. Azim
Lumley, Sheila F.
Littlejohn, Margaret
Revill, Peter
McKeating, Jane A.
Matthews, Philippa C.
author_sort McNaughton, Anna L.
collection PubMed
description Hepatitis B virus (HBV) is a unique, tiny, partially double-stranded, reverse-transcribing DNA virus with proteins encoded by multiple overlapping reading frames. The substitution rate is surprisingly high for a DNA virus, but lower than that of other reverse transcribing organisms. More than 260 million people worldwide have chronic HBV infection, which causes 0.8 million deaths a year. Because of the high burden of disease, international health agencies have set the goal of eliminating HBV infection by 2030. Nonetheless, the intriguing HBV genome has not been well characterized. We summarize data on the HBV genome structure and replication cycle, explain and quantify diversity within and among infected individuals, and discuss advances that can be offered by application of next-generation sequencing technology. In-depth HBV genome analyses could increase our understanding of disease pathogenesis and allow us to better predict patient outcomes, optimize treatment, and develop new therapeutics.
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spelling pubmed-63475712019-01-29 Insights From Deep Sequencing of the HBV Genome—Unique, Tiny, and Misunderstood McNaughton, Anna L. D’Arienzo, Valentina Ansari, M. Azim Lumley, Sheila F. Littlejohn, Margaret Revill, Peter McKeating, Jane A. Matthews, Philippa C. Gastroenterology Article Hepatitis B virus (HBV) is a unique, tiny, partially double-stranded, reverse-transcribing DNA virus with proteins encoded by multiple overlapping reading frames. The substitution rate is surprisingly high for a DNA virus, but lower than that of other reverse transcribing organisms. More than 260 million people worldwide have chronic HBV infection, which causes 0.8 million deaths a year. Because of the high burden of disease, international health agencies have set the goal of eliminating HBV infection by 2030. Nonetheless, the intriguing HBV genome has not been well characterized. We summarize data on the HBV genome structure and replication cycle, explain and quantify diversity within and among infected individuals, and discuss advances that can be offered by application of next-generation sequencing technology. In-depth HBV genome analyses could increase our understanding of disease pathogenesis and allow us to better predict patient outcomes, optimize treatment, and develop new therapeutics. by the AGA Institute. Published by Elsevier Inc. 2019-01 2018-09-27 /pmc/articles/PMC6347571/ /pubmed/30268787 http://dx.doi.org/10.1053/j.gastro.2018.07.058 Text en © 2019 by the AGA Institute. Published by Elsevier Inc. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
McNaughton, Anna L.
D’Arienzo, Valentina
Ansari, M. Azim
Lumley, Sheila F.
Littlejohn, Margaret
Revill, Peter
McKeating, Jane A.
Matthews, Philippa C.
Insights From Deep Sequencing of the HBV Genome—Unique, Tiny, and Misunderstood
title Insights From Deep Sequencing of the HBV Genome—Unique, Tiny, and Misunderstood
title_full Insights From Deep Sequencing of the HBV Genome—Unique, Tiny, and Misunderstood
title_fullStr Insights From Deep Sequencing of the HBV Genome—Unique, Tiny, and Misunderstood
title_full_unstemmed Insights From Deep Sequencing of the HBV Genome—Unique, Tiny, and Misunderstood
title_short Insights From Deep Sequencing of the HBV Genome—Unique, Tiny, and Misunderstood
title_sort insights from deep sequencing of the hbv genome—unique, tiny, and misunderstood
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6347571/
https://www.ncbi.nlm.nih.gov/pubmed/30268787
http://dx.doi.org/10.1053/j.gastro.2018.07.058
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