High-efficiency expression and secretion of human FGF21 in Bacillus subtilis by intercalation of a mini-cistron cassette and combinatorial optimization of cell regulatory components

BACKGROUND: Recombinant human Fibroblast growth factor 21 (rhFGF21) is an endocrine hormone that has profound effects on treatment of metabolic diseases. However, rhFGF21 is prone to form inclusion body when expressed in bacteria, which results in, the downstream process of purification of bioactive...

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Detalles Bibliográficos
Autores principales: Li, Dandan, Fu, Gang, Tu, Ran, Jin, Zhaoxia, Zhang, Dawei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2019
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6348689/
https://www.ncbi.nlm.nih.gov/pubmed/30691455
http://dx.doi.org/10.1186/s12934-019-1066-4

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6348689/
https://www.ncbi.nlm.nih.gov/pubmed/30691455
http://dx.doi.org/10.1186/s12934-019-1066-4

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