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FGF21 trafficking in intact human cells revealed by cryo-electron tomography with gold nanoparticles
The fibroblast growth factor FGF21 was labeled with molecularly defined gold nanoparticles (AuNPs), applied to human adipocytes, and imaged by cryo-electron tomography (cryo-ET). Most AuNPs were in pairs about 80 Å apart, on the outer cell surface. Pairs of AuNPs were also abundant inside the cells...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
eLife Sciences Publications, Ltd
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6349402/ https://www.ncbi.nlm.nih.gov/pubmed/30688648 http://dx.doi.org/10.7554/eLife.43146 |
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author | Azubel, Maia Carter, Stephen D Weiszmann, Jennifer Zhang, Jun Jensen, Grant J Li, Yang Kornberg, Roger D |
author_facet | Azubel, Maia Carter, Stephen D Weiszmann, Jennifer Zhang, Jun Jensen, Grant J Li, Yang Kornberg, Roger D |
author_sort | Azubel, Maia |
collection | PubMed |
description | The fibroblast growth factor FGF21 was labeled with molecularly defined gold nanoparticles (AuNPs), applied to human adipocytes, and imaged by cryo-electron tomography (cryo-ET). Most AuNPs were in pairs about 80 Å apart, on the outer cell surface. Pairs of AuNPs were also abundant inside the cells in clathrin-coated vesicles and endosomes. AuNPs were present but no longer paired in multivesicular bodies. FGF21 could thus be tracked along the endocytotic pathway. The methods developed here to visualize signaling coupled to endocytosis can be applied to a wide variety of cargo and may be extended to studies of other intracellular transactions. |
format | Online Article Text |
id | pubmed-6349402 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | eLife Sciences Publications, Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-63494022019-01-30 FGF21 trafficking in intact human cells revealed by cryo-electron tomography with gold nanoparticles Azubel, Maia Carter, Stephen D Weiszmann, Jennifer Zhang, Jun Jensen, Grant J Li, Yang Kornberg, Roger D eLife Cell Biology The fibroblast growth factor FGF21 was labeled with molecularly defined gold nanoparticles (AuNPs), applied to human adipocytes, and imaged by cryo-electron tomography (cryo-ET). Most AuNPs were in pairs about 80 Å apart, on the outer cell surface. Pairs of AuNPs were also abundant inside the cells in clathrin-coated vesicles and endosomes. AuNPs were present but no longer paired in multivesicular bodies. FGF21 could thus be tracked along the endocytotic pathway. The methods developed here to visualize signaling coupled to endocytosis can be applied to a wide variety of cargo and may be extended to studies of other intracellular transactions. eLife Sciences Publications, Ltd 2019-01-28 /pmc/articles/PMC6349402/ /pubmed/30688648 http://dx.doi.org/10.7554/eLife.43146 Text en © 2019, Azubel et al http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited. |
spellingShingle | Cell Biology Azubel, Maia Carter, Stephen D Weiszmann, Jennifer Zhang, Jun Jensen, Grant J Li, Yang Kornberg, Roger D FGF21 trafficking in intact human cells revealed by cryo-electron tomography with gold nanoparticles |
title | FGF21 trafficking in intact human cells revealed by cryo-electron tomography with gold nanoparticles |
title_full | FGF21 trafficking in intact human cells revealed by cryo-electron tomography with gold nanoparticles |
title_fullStr | FGF21 trafficking in intact human cells revealed by cryo-electron tomography with gold nanoparticles |
title_full_unstemmed | FGF21 trafficking in intact human cells revealed by cryo-electron tomography with gold nanoparticles |
title_short | FGF21 trafficking in intact human cells revealed by cryo-electron tomography with gold nanoparticles |
title_sort | fgf21 trafficking in intact human cells revealed by cryo-electron tomography with gold nanoparticles |
topic | Cell Biology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6349402/ https://www.ncbi.nlm.nih.gov/pubmed/30688648 http://dx.doi.org/10.7554/eLife.43146 |
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