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FGF21 trafficking in intact human cells revealed by cryo-electron tomography with gold nanoparticles

The fibroblast growth factor FGF21 was labeled with molecularly defined gold nanoparticles (AuNPs), applied to human adipocytes, and imaged by cryo-electron tomography (cryo-ET). Most AuNPs were in pairs about 80 Å apart, on the outer cell surface. Pairs of AuNPs were also abundant inside the cells...

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Autores principales: Azubel, Maia, Carter, Stephen D, Weiszmann, Jennifer, Zhang, Jun, Jensen, Grant J, Li, Yang, Kornberg, Roger D
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6349402/
https://www.ncbi.nlm.nih.gov/pubmed/30688648
http://dx.doi.org/10.7554/eLife.43146
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author Azubel, Maia
Carter, Stephen D
Weiszmann, Jennifer
Zhang, Jun
Jensen, Grant J
Li, Yang
Kornberg, Roger D
author_facet Azubel, Maia
Carter, Stephen D
Weiszmann, Jennifer
Zhang, Jun
Jensen, Grant J
Li, Yang
Kornberg, Roger D
author_sort Azubel, Maia
collection PubMed
description The fibroblast growth factor FGF21 was labeled with molecularly defined gold nanoparticles (AuNPs), applied to human adipocytes, and imaged by cryo-electron tomography (cryo-ET). Most AuNPs were in pairs about 80 Å apart, on the outer cell surface. Pairs of AuNPs were also abundant inside the cells in clathrin-coated vesicles and endosomes. AuNPs were present but no longer paired in multivesicular bodies. FGF21 could thus be tracked along the endocytotic pathway. The methods developed here to visualize signaling coupled to endocytosis can be applied to a wide variety of cargo and may be extended to studies of other intracellular transactions.
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spelling pubmed-63494022019-01-30 FGF21 trafficking in intact human cells revealed by cryo-electron tomography with gold nanoparticles Azubel, Maia Carter, Stephen D Weiszmann, Jennifer Zhang, Jun Jensen, Grant J Li, Yang Kornberg, Roger D eLife Cell Biology The fibroblast growth factor FGF21 was labeled with molecularly defined gold nanoparticles (AuNPs), applied to human adipocytes, and imaged by cryo-electron tomography (cryo-ET). Most AuNPs were in pairs about 80 Å apart, on the outer cell surface. Pairs of AuNPs were also abundant inside the cells in clathrin-coated vesicles and endosomes. AuNPs were present but no longer paired in multivesicular bodies. FGF21 could thus be tracked along the endocytotic pathway. The methods developed here to visualize signaling coupled to endocytosis can be applied to a wide variety of cargo and may be extended to studies of other intracellular transactions. eLife Sciences Publications, Ltd 2019-01-28 /pmc/articles/PMC6349402/ /pubmed/30688648 http://dx.doi.org/10.7554/eLife.43146 Text en © 2019, Azubel et al http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Cell Biology
Azubel, Maia
Carter, Stephen D
Weiszmann, Jennifer
Zhang, Jun
Jensen, Grant J
Li, Yang
Kornberg, Roger D
FGF21 trafficking in intact human cells revealed by cryo-electron tomography with gold nanoparticles
title FGF21 trafficking in intact human cells revealed by cryo-electron tomography with gold nanoparticles
title_full FGF21 trafficking in intact human cells revealed by cryo-electron tomography with gold nanoparticles
title_fullStr FGF21 trafficking in intact human cells revealed by cryo-electron tomography with gold nanoparticles
title_full_unstemmed FGF21 trafficking in intact human cells revealed by cryo-electron tomography with gold nanoparticles
title_short FGF21 trafficking in intact human cells revealed by cryo-electron tomography with gold nanoparticles
title_sort fgf21 trafficking in intact human cells revealed by cryo-electron tomography with gold nanoparticles
topic Cell Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6349402/
https://www.ncbi.nlm.nih.gov/pubmed/30688648
http://dx.doi.org/10.7554/eLife.43146
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