Development of a peptide-based fluorescent probe for biological heme monitoring

Heme plays a vital role in cell biology and dysregulation of heme levels is implicated in a wide range of diseases. However, monitoring heme levels in biological systems is currently not straightforward. A short synthetic peptide probe containing 7-azatryptophan is shown to bind hemin in vitro with...

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Detalles Bibliográficos
Autores principales: Newton, Laura D., Pascu, Sofia I., Tyrrell, Rex M., Eggleston, Ian M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Royal Society of Chemistry 2019
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6350759/
https://www.ncbi.nlm.nih.gov/pubmed/30574967
http://dx.doi.org/10.1039/c8ob02290a
Descripción
Sumario:Heme plays a vital role in cell biology and dysregulation of heme levels is implicated in a wide range of diseases. However, monitoring heme levels in biological systems is currently not straightforward. A short synthetic peptide probe containing 7-azatryptophan is shown to bind hemin in vitro with quenching of the azatryptophan fluorescence. This chemical tool can be used to detect the change in free heme induced in human skin cells upon exposure to UVA irradiation.

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