Development of a peptide-based fluorescent probe for biological heme monitoring

Heme plays a vital role in cell biology and dysregulation of heme levels is implicated in a wide range of diseases. However, monitoring heme levels in biological systems is currently not straightforward. A short synthetic peptide probe containing 7-azatryptophan is shown to bind hemin in vitro with...

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Detalles Bibliográficos
Autores principales: Newton, Laura D., Pascu, Sofia I., Tyrrell, Rex M., Eggleston, Ian M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Royal Society of Chemistry 2019
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6350759/
https://www.ncbi.nlm.nih.gov/pubmed/30574967
http://dx.doi.org/10.1039/c8ob02290a
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author Newton, Laura D.
Pascu, Sofia I.
Tyrrell, Rex M.
Eggleston, Ian M.
author_facet Newton, Laura D.
Pascu, Sofia I.
Tyrrell, Rex M.
Eggleston, Ian M.
author_sort Newton, Laura D.
collection PubMed
description Heme plays a vital role in cell biology and dysregulation of heme levels is implicated in a wide range of diseases. However, monitoring heme levels in biological systems is currently not straightforward. A short synthetic peptide probe containing 7-azatryptophan is shown to bind hemin in vitro with quenching of the azatryptophan fluorescence. This chemical tool can be used to detect the change in free heme induced in human skin cells upon exposure to UVA irradiation.
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spelling pubmed-63507592019-02-15 Development of a peptide-based fluorescent probe for biological heme monitoring Newton, Laura D. Pascu, Sofia I. Tyrrell, Rex M. Eggleston, Ian M. Org Biomol Chem Chemistry Heme plays a vital role in cell biology and dysregulation of heme levels is implicated in a wide range of diseases. However, monitoring heme levels in biological systems is currently not straightforward. A short synthetic peptide probe containing 7-azatryptophan is shown to bind hemin in vitro with quenching of the azatryptophan fluorescence. This chemical tool can be used to detect the change in free heme induced in human skin cells upon exposure to UVA irradiation. Royal Society of Chemistry 2019-01-21 2018-12-18 /pmc/articles/PMC6350759/ /pubmed/30574967 http://dx.doi.org/10.1039/c8ob02290a Text en This journal is © The Royal Society of Chemistry 2019 http://creativecommons.org/licenses/by/3.0/ This article is freely available. This article is licensed under a Creative Commons Attribution 3.0 Unported Licence (CC BY 3.0)
spellingShingle Chemistry
Newton, Laura D.
Pascu, Sofia I.
Tyrrell, Rex M.
Eggleston, Ian M.
Development of a peptide-based fluorescent probe for biological heme monitoring
title Development of a peptide-based fluorescent probe for biological heme monitoring
title_full Development of a peptide-based fluorescent probe for biological heme monitoring
title_fullStr Development of a peptide-based fluorescent probe for biological heme monitoring
title_full_unstemmed Development of a peptide-based fluorescent probe for biological heme monitoring
title_short Development of a peptide-based fluorescent probe for biological heme monitoring
title_sort development of a peptide-based fluorescent probe for biological heme monitoring
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6350759/
https://www.ncbi.nlm.nih.gov/pubmed/30574967
http://dx.doi.org/10.1039/c8ob02290a
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