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Employment of L. paracasei K5 as a Novel Potentially Probiotic Freeze-Dried Starter for Feta-Type Cheese Production

In the present study, a novel potentially probiotic Lactobacillus paracasei strain, previously isolated from dairy products, was evaluated as a starter culture of Feta-type cheese production. Targeting industrial applications, the starter culture was applied as a ready-to-use freeze-dried culture th...

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Autores principales: Terpou, Antonia, Mantzourani, Ioanna, Galanis, Alex, Kanellaki, Maria, Bezirtzoglou, Eugenia, Bekatorou, Argyro, Koutinas, Athanasios A., Plessas, Stavros
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6352075/
https://www.ncbi.nlm.nih.gov/pubmed/30587786
http://dx.doi.org/10.3390/microorganisms7010003
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author Terpou, Antonia
Mantzourani, Ioanna
Galanis, Alex
Kanellaki, Maria
Bezirtzoglou, Eugenia
Bekatorou, Argyro
Koutinas, Athanasios A.
Plessas, Stavros
author_facet Terpou, Antonia
Mantzourani, Ioanna
Galanis, Alex
Kanellaki, Maria
Bezirtzoglou, Eugenia
Bekatorou, Argyro
Koutinas, Athanasios A.
Plessas, Stavros
author_sort Terpou, Antonia
collection PubMed
description In the present study, a novel potentially probiotic Lactobacillus paracasei strain, previously isolated from dairy products, was evaluated as a starter culture of Feta-type cheese production. Targeting industrial applications, the starter culture was applied as a ready-to-use freeze-dried culture that was either free or immobilized. The immobilized biocatalyst composed of Lactobacillus paracasei K5 cells absorbed within delignified wheat bran prebiotic carrier. All produced cheeses were compared with cheese manufactured by renin enzyme. Several parameters that affect acceptability, quality and shelf-life of Feta-type cheese were investigated, including microbial populations, physicochemical characteristics and cheese volatiles through 90 days of ripening and storage. Survival of L. paracasei K5 remained in high levels (≥6.0 log cfu/g) after the 90th day of cheese production, as recorded by combining microbiological enumeration and strain-specific multiplex PCR analysis. The use of the freeze-dried novel starter culture (free or immobilized) enhanced the aromatic profile of Feta-type cheeses. Finally, the use of the potentially synbiotic immobilized biocatalyst further improved aromatic characteristics of produced cheese and decrease of possible spoilage or pathogenic microorganisms. These findings indicate the potential industrial use of freeze-dried L. paracasei K5 as starter culture for the production of good-quality functional Feta-type cheese.
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spelling pubmed-63520752019-02-01 Employment of L. paracasei K5 as a Novel Potentially Probiotic Freeze-Dried Starter for Feta-Type Cheese Production Terpou, Antonia Mantzourani, Ioanna Galanis, Alex Kanellaki, Maria Bezirtzoglou, Eugenia Bekatorou, Argyro Koutinas, Athanasios A. Plessas, Stavros Microorganisms Article In the present study, a novel potentially probiotic Lactobacillus paracasei strain, previously isolated from dairy products, was evaluated as a starter culture of Feta-type cheese production. Targeting industrial applications, the starter culture was applied as a ready-to-use freeze-dried culture that was either free or immobilized. The immobilized biocatalyst composed of Lactobacillus paracasei K5 cells absorbed within delignified wheat bran prebiotic carrier. All produced cheeses were compared with cheese manufactured by renin enzyme. Several parameters that affect acceptability, quality and shelf-life of Feta-type cheese were investigated, including microbial populations, physicochemical characteristics and cheese volatiles through 90 days of ripening and storage. Survival of L. paracasei K5 remained in high levels (≥6.0 log cfu/g) after the 90th day of cheese production, as recorded by combining microbiological enumeration and strain-specific multiplex PCR analysis. The use of the freeze-dried novel starter culture (free or immobilized) enhanced the aromatic profile of Feta-type cheeses. Finally, the use of the potentially synbiotic immobilized biocatalyst further improved aromatic characteristics of produced cheese and decrease of possible spoilage or pathogenic microorganisms. These findings indicate the potential industrial use of freeze-dried L. paracasei K5 as starter culture for the production of good-quality functional Feta-type cheese. MDPI 2018-12-26 /pmc/articles/PMC6352075/ /pubmed/30587786 http://dx.doi.org/10.3390/microorganisms7010003 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Terpou, Antonia
Mantzourani, Ioanna
Galanis, Alex
Kanellaki, Maria
Bezirtzoglou, Eugenia
Bekatorou, Argyro
Koutinas, Athanasios A.
Plessas, Stavros
Employment of L. paracasei K5 as a Novel Potentially Probiotic Freeze-Dried Starter for Feta-Type Cheese Production
title Employment of L. paracasei K5 as a Novel Potentially Probiotic Freeze-Dried Starter for Feta-Type Cheese Production
title_full Employment of L. paracasei K5 as a Novel Potentially Probiotic Freeze-Dried Starter for Feta-Type Cheese Production
title_fullStr Employment of L. paracasei K5 as a Novel Potentially Probiotic Freeze-Dried Starter for Feta-Type Cheese Production
title_full_unstemmed Employment of L. paracasei K5 as a Novel Potentially Probiotic Freeze-Dried Starter for Feta-Type Cheese Production
title_short Employment of L. paracasei K5 as a Novel Potentially Probiotic Freeze-Dried Starter for Feta-Type Cheese Production
title_sort employment of l. paracasei k5 as a novel potentially probiotic freeze-dried starter for feta-type cheese production
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6352075/
https://www.ncbi.nlm.nih.gov/pubmed/30587786
http://dx.doi.org/10.3390/microorganisms7010003
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