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Miniaturisation of high-throughput plasmid DNA library preparation for next-generation sequencing using multifactorial optimisation

High-throughput preparation of plasmid DNA libraries for next-generation sequencing (NGS) is an important capability for molecular biology laboratories. In particular, it is an essential quality control (QC) check when large numbers of plasmid variants are being generated. Here, we describe the use...

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Detalles Bibliográficos
Autores principales: Suckling, Lorna, McFarlane, Ciaran, Sawyer, Chelsea, Chambers, Stephen P., Kitney, Richard I., McClymont, David W., Freemont, Paul S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: KeAi Publishing 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6352296/
https://www.ncbi.nlm.nih.gov/pubmed/30723818
http://dx.doi.org/10.1016/j.synbio.2019.01.002