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Miniaturisation of high-throughput plasmid DNA library preparation for next-generation sequencing using multifactorial optimisation
High-throughput preparation of plasmid DNA libraries for next-generation sequencing (NGS) is an important capability for molecular biology laboratories. In particular, it is an essential quality control (QC) check when large numbers of plasmid variants are being generated. Here, we describe the use...
Autores principales: | Suckling, Lorna, McFarlane, Ciaran, Sawyer, Chelsea, Chambers, Stephen P., Kitney, Richard I., McClymont, David W., Freemont, Paul S. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
KeAi Publishing
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6352296/ https://www.ncbi.nlm.nih.gov/pubmed/30723818 http://dx.doi.org/10.1016/j.synbio.2019.01.002 |
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