sgRNA Sequence Motifs Blocking Efficient CRISPR/Cas9-Mediated Gene Editing

Cas9 nucleases can be programmed with single guide RNAs (sgRNAs) to mediate gene editing. High CRISPR/Cas9-mediated gene knockout efficiencies are essential for genetic screens and critically depend on the properties of the sgRNAs used. The specificity of an sgRNA is defined by its targeting sequenc...

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Detalles Bibliográficos
Autores principales: Graf, Robin, Li, Xun, Chu, Van Trung, Rajewsky, Klaus
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cell Press 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6352712/
https://www.ncbi.nlm.nih.gov/pubmed/30699341
http://dx.doi.org/10.1016/j.celrep.2019.01.024

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6352712/
https://www.ncbi.nlm.nih.gov/pubmed/30699341
http://dx.doi.org/10.1016/j.celrep.2019.01.024

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