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sgRNA Sequence Motifs Blocking Efficient CRISPR/Cas9-Mediated Gene Editing
Cas9 nucleases can be programmed with single guide RNAs (sgRNAs) to mediate gene editing. High CRISPR/Cas9-mediated gene knockout efficiencies are essential for genetic screens and critically depend on the properties of the sgRNAs used. The specificity of an sgRNA is defined by its targeting sequenc...
| Autores principales: | , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Cell Press
2019
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6352712/ https://www.ncbi.nlm.nih.gov/pubmed/30699341 http://dx.doi.org/10.1016/j.celrep.2019.01.024 |