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Quantitative monitoring of the cytoplasmic release of NCp7 proteins from individual HIV-1 viral cores during the early steps of infection

Fluorescence microscopy imaging of individual HIV-1 viruses necessitates a specific labeling of viral structures that minimally perturbs the infection process. Herein, we used HIV-1 pseudoviruses containing NCp7 fused to a tetracystein (TC) tag, labeled by a biarsenical fluorescein derivative (FlAsH...

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Detalles Bibliográficos
Autores principales: Zgheib, Sarwat, Lysova, Iryna, Réal, Eleonore, Dukhno, Oleksii, Vauchelles, Romain, Pires, Manuel, Anton, Halina, Mély, Yves
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6353972/
https://www.ncbi.nlm.nih.gov/pubmed/30700731
http://dx.doi.org/10.1038/s41598-018-37150-0