Genetic Diversity and Phylodynamics of Avian Coronaviruses in Egyptian Wild Birds

Avian coronaviruses (ACoVs) are continuously evolving and causing serious economic consequences in the poultry industry and around the globe. Owing to their extensive genetic diversity and high mutation rates, controlling ACoVs has become a challenge. In this context, the potential contribution of w...

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Autores principales: A. Rohaim, Mohammed, F. El Naggar, Rania, M. Helal, Ahmed, M. Bayoumi, Mahmoud, A. El-Saied, Mohamed, A. Ahmed, Kawkab, Z. Shabbir, Muhammad, Munir, Muhammad
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6356246/
https://www.ncbi.nlm.nih.gov/pubmed/30646528
http://dx.doi.org/10.3390/v11010057
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author A. Rohaim, Mohammed
F. El Naggar, Rania
M. Helal, Ahmed
M. Bayoumi, Mahmoud
A. El-Saied, Mohamed
A. Ahmed, Kawkab
Z. Shabbir, Muhammad
Munir, Muhammad
author_facet A. Rohaim, Mohammed
F. El Naggar, Rania
M. Helal, Ahmed
M. Bayoumi, Mahmoud
A. El-Saied, Mohamed
A. Ahmed, Kawkab
Z. Shabbir, Muhammad
Munir, Muhammad
author_sort A. Rohaim, Mohammed
collection PubMed
description Avian coronaviruses (ACoVs) are continuously evolving and causing serious economic consequences in the poultry industry and around the globe. Owing to their extensive genetic diversity and high mutation rates, controlling ACoVs has become a challenge. In this context, the potential contribution of wild birds in the disease dynamics, especially in domesticated birds, remains largely unknown. In the present study, five hundred fifty-seven (n = 557) cloacal/fecal swabs were collected from four different wild bird species from eight Egyptian governorates during 2016 and a total of fourteen positive isolates were used for phylodynamics and evolutionary analysis. Genetic relatedness based on spike (S1) gene demonstrated the clustering of majority of these isolates where nine isolates grouped within Egy/variant 2 (IS/885 genotype) and five isolates clustered within Egy/variant 1 (IS/1494/06 genotype). Interestingly, these isolates showed noticeable genetic diversity and were clustered distal to the previously characterized Egy/variant 1 and Egy/variant 2 in Egyptian commercial poultry. The S1 gene based comparison of nucleotide identity percentages revealed that all fourteen isolates reported in this study were genetically related to the variant GI-23 lineage with 92–100% identity. Taken together, our results demonstrate that ACoVs are circulating in Egyptian wild birds and highlight their possible contributions in the disease dynamics. The study also proposes that regular monitoring of the ACoVs in wild birds is required to effectively assess the role of wild birds in disease spread, and the emergence of ACoVs strains in the country.
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spelling pubmed-63562462019-02-05 Genetic Diversity and Phylodynamics of Avian Coronaviruses in Egyptian Wild Birds A. Rohaim, Mohammed F. El Naggar, Rania M. Helal, Ahmed M. Bayoumi, Mahmoud A. El-Saied, Mohamed A. Ahmed, Kawkab Z. Shabbir, Muhammad Munir, Muhammad Viruses Article Avian coronaviruses (ACoVs) are continuously evolving and causing serious economic consequences in the poultry industry and around the globe. Owing to their extensive genetic diversity and high mutation rates, controlling ACoVs has become a challenge. In this context, the potential contribution of wild birds in the disease dynamics, especially in domesticated birds, remains largely unknown. In the present study, five hundred fifty-seven (n = 557) cloacal/fecal swabs were collected from four different wild bird species from eight Egyptian governorates during 2016 and a total of fourteen positive isolates were used for phylodynamics and evolutionary analysis. Genetic relatedness based on spike (S1) gene demonstrated the clustering of majority of these isolates where nine isolates grouped within Egy/variant 2 (IS/885 genotype) and five isolates clustered within Egy/variant 1 (IS/1494/06 genotype). Interestingly, these isolates showed noticeable genetic diversity and were clustered distal to the previously characterized Egy/variant 1 and Egy/variant 2 in Egyptian commercial poultry. The S1 gene based comparison of nucleotide identity percentages revealed that all fourteen isolates reported in this study were genetically related to the variant GI-23 lineage with 92–100% identity. Taken together, our results demonstrate that ACoVs are circulating in Egyptian wild birds and highlight their possible contributions in the disease dynamics. The study also proposes that regular monitoring of the ACoVs in wild birds is required to effectively assess the role of wild birds in disease spread, and the emergence of ACoVs strains in the country. MDPI 2019-01-14 /pmc/articles/PMC6356246/ /pubmed/30646528 http://dx.doi.org/10.3390/v11010057 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
A. Rohaim, Mohammed
F. El Naggar, Rania
M. Helal, Ahmed
M. Bayoumi, Mahmoud
A. El-Saied, Mohamed
A. Ahmed, Kawkab
Z. Shabbir, Muhammad
Munir, Muhammad
Genetic Diversity and Phylodynamics of Avian Coronaviruses in Egyptian Wild Birds
title Genetic Diversity and Phylodynamics of Avian Coronaviruses in Egyptian Wild Birds
title_full Genetic Diversity and Phylodynamics of Avian Coronaviruses in Egyptian Wild Birds
title_fullStr Genetic Diversity and Phylodynamics of Avian Coronaviruses in Egyptian Wild Birds
title_full_unstemmed Genetic Diversity and Phylodynamics of Avian Coronaviruses in Egyptian Wild Birds
title_short Genetic Diversity and Phylodynamics of Avian Coronaviruses in Egyptian Wild Birds
title_sort genetic diversity and phylodynamics of avian coronaviruses in egyptian wild birds
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6356246/
https://www.ncbi.nlm.nih.gov/pubmed/30646528
http://dx.doi.org/10.3390/v11010057
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