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Ethanol Conditioned Taste Aversion in High Drinking in the Dark Mice

Two independent lines of High Drinking in the Dark (HDID-1, HDID-2) mice have been bred to reach high blood alcohol levels after a short period of binge-like ethanol drinking. Male mice of both lines were shown to have reduced sensitivity to develop a taste aversion to a novel flavor conditioned by...

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Autores principales: Crabbe, John C., Metten, Pamela, Savarese, Antonia M., Ozburn, Angela R., Schlumbohm, Jason P., Spence, Stephanie E., Hack, Wyatt R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6356868/
https://www.ncbi.nlm.nih.gov/pubmed/30609665
http://dx.doi.org/10.3390/brainsci9010002
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author Crabbe, John C.
Metten, Pamela
Savarese, Antonia M.
Ozburn, Angela R.
Schlumbohm, Jason P.
Spence, Stephanie E.
Hack, Wyatt R.
author_facet Crabbe, John C.
Metten, Pamela
Savarese, Antonia M.
Ozburn, Angela R.
Schlumbohm, Jason P.
Spence, Stephanie E.
Hack, Wyatt R.
author_sort Crabbe, John C.
collection PubMed
description Two independent lines of High Drinking in the Dark (HDID-1, HDID-2) mice have been bred to reach high blood alcohol levels after a short period of binge-like ethanol drinking. Male mice of both lines were shown to have reduced sensitivity to develop a taste aversion to a novel flavor conditioned by ethanol injections as compared with their unselected HS/NPT founder stock. We have subsequently developed inbred variants of each line. The current experiments established that reduced ethanol-conditioned taste aversion is also seen in the inbred variants, in both males and females. In other experiments, we asked whether HDID mice would ingest sufficient doses of ethanol to lead to a conditioned taste aversion upon retest. Different manipulations were used to elevate consumption of ethanol on initial exposure. Access to increased ethanol concentrations, to multiple tubes of ethanol, and fluid restriction to increase thirst motivation all enhanced initial drinking of ethanol. Each condition led to reduced intake the next day, consistent with a mild conditioned taste aversion. These experiments support the conclusion that one reason contributing to the willingness of HDID mice to drink to the point of intoxication is a genetic insensitivity to the aversive effects of ethanol.
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spelling pubmed-63568682019-02-05 Ethanol Conditioned Taste Aversion in High Drinking in the Dark Mice Crabbe, John C. Metten, Pamela Savarese, Antonia M. Ozburn, Angela R. Schlumbohm, Jason P. Spence, Stephanie E. Hack, Wyatt R. Brain Sci Article Two independent lines of High Drinking in the Dark (HDID-1, HDID-2) mice have been bred to reach high blood alcohol levels after a short period of binge-like ethanol drinking. Male mice of both lines were shown to have reduced sensitivity to develop a taste aversion to a novel flavor conditioned by ethanol injections as compared with their unselected HS/NPT founder stock. We have subsequently developed inbred variants of each line. The current experiments established that reduced ethanol-conditioned taste aversion is also seen in the inbred variants, in both males and females. In other experiments, we asked whether HDID mice would ingest sufficient doses of ethanol to lead to a conditioned taste aversion upon retest. Different manipulations were used to elevate consumption of ethanol on initial exposure. Access to increased ethanol concentrations, to multiple tubes of ethanol, and fluid restriction to increase thirst motivation all enhanced initial drinking of ethanol. Each condition led to reduced intake the next day, consistent with a mild conditioned taste aversion. These experiments support the conclusion that one reason contributing to the willingness of HDID mice to drink to the point of intoxication is a genetic insensitivity to the aversive effects of ethanol. MDPI 2019-01-01 /pmc/articles/PMC6356868/ /pubmed/30609665 http://dx.doi.org/10.3390/brainsci9010002 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Crabbe, John C.
Metten, Pamela
Savarese, Antonia M.
Ozburn, Angela R.
Schlumbohm, Jason P.
Spence, Stephanie E.
Hack, Wyatt R.
Ethanol Conditioned Taste Aversion in High Drinking in the Dark Mice
title Ethanol Conditioned Taste Aversion in High Drinking in the Dark Mice
title_full Ethanol Conditioned Taste Aversion in High Drinking in the Dark Mice
title_fullStr Ethanol Conditioned Taste Aversion in High Drinking in the Dark Mice
title_full_unstemmed Ethanol Conditioned Taste Aversion in High Drinking in the Dark Mice
title_short Ethanol Conditioned Taste Aversion in High Drinking in the Dark Mice
title_sort ethanol conditioned taste aversion in high drinking in the dark mice
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6356868/
https://www.ncbi.nlm.nih.gov/pubmed/30609665
http://dx.doi.org/10.3390/brainsci9010002
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