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Effects of Dietary Protein and Fat Content on Intrahepatocellular and Intramyocellular Lipids during a 6-Day Hypercaloric, High Sucrose Diet: A Randomized Controlled Trial in Normal Weight Healthy Subjects

Sucrose overfeeding increases intrahepatocellular (IHCL) and intramyocellular (IMCL) lipid concentrations in healthy subjects. We hypothesized that these effects would be modulated by diet protein/fat content. Twelve healthy men and women were studied on two occasions in a randomized, cross-over tri...

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Autores principales: Surowska, Anna, Jegatheesan, Prasanthi, Campos, Vanessa, Marques, Anne-Sophie, Egli, Léonie, Cros, Jérémy, Rosset, Robin, Lecoultre, Virgile, Kreis, Roland, Boesch, Chris, Pouymayou, Bertrand, Schneiter, Philippe, Tappy, Luc
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6357079/
https://www.ncbi.nlm.nih.gov/pubmed/30669704
http://dx.doi.org/10.3390/nu11010209
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author Surowska, Anna
Jegatheesan, Prasanthi
Campos, Vanessa
Marques, Anne-Sophie
Egli, Léonie
Cros, Jérémy
Rosset, Robin
Lecoultre, Virgile
Kreis, Roland
Boesch, Chris
Pouymayou, Bertrand
Schneiter, Philippe
Tappy, Luc
author_facet Surowska, Anna
Jegatheesan, Prasanthi
Campos, Vanessa
Marques, Anne-Sophie
Egli, Léonie
Cros, Jérémy
Rosset, Robin
Lecoultre, Virgile
Kreis, Roland
Boesch, Chris
Pouymayou, Bertrand
Schneiter, Philippe
Tappy, Luc
author_sort Surowska, Anna
collection PubMed
description Sucrose overfeeding increases intrahepatocellular (IHCL) and intramyocellular (IMCL) lipid concentrations in healthy subjects. We hypothesized that these effects would be modulated by diet protein/fat content. Twelve healthy men and women were studied on two occasions in a randomized, cross-over trial. On each occasion, they received a 3-day 12% protein weight maintenance diet (WM) followed by a 6-day hypercaloric high sucrose diet (150% energy requirements). On one occasion the hypercaloric diet contained 5% protein and 25% fat (low protein-high fat, LP-HF), on the other occasion it contained 20% protein and 10% fat (high protein-low fat, HP-LF). IHCL and IMCL concentrations (magnetic resonance spectroscopy) and energy expenditure (indirect calorimetry) were measured after WM, and again after HP-LF/LP-HF. IHCL increased from 25.0 ± 3.6 after WM to 147.1 ± 26.9 mmol/kg wet weight (ww) after LP-HF and from 30.3 ± 7.7 to 57.8 ± 14.8 after HP-LF (two-way ANOVA with interaction: p < 0.001 overfeeding x protein/fat content). IMCL increased from 7.1 ± 0.6 to 8.8 ± 0.7 mmol/kg ww after LP-HF and from 6.2 ± 0.6 to 6.9 ± 0.6 after HP-LF, (p < 0.002). These results indicate that liver and muscle fat deposition is enhanced when sucrose overfeeding is associated with a low protein, high fat diet compared to a high protein, low fat diet.
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spelling pubmed-63570792019-02-04 Effects of Dietary Protein and Fat Content on Intrahepatocellular and Intramyocellular Lipids during a 6-Day Hypercaloric, High Sucrose Diet: A Randomized Controlled Trial in Normal Weight Healthy Subjects Surowska, Anna Jegatheesan, Prasanthi Campos, Vanessa Marques, Anne-Sophie Egli, Léonie Cros, Jérémy Rosset, Robin Lecoultre, Virgile Kreis, Roland Boesch, Chris Pouymayou, Bertrand Schneiter, Philippe Tappy, Luc Nutrients Article Sucrose overfeeding increases intrahepatocellular (IHCL) and intramyocellular (IMCL) lipid concentrations in healthy subjects. We hypothesized that these effects would be modulated by diet protein/fat content. Twelve healthy men and women were studied on two occasions in a randomized, cross-over trial. On each occasion, they received a 3-day 12% protein weight maintenance diet (WM) followed by a 6-day hypercaloric high sucrose diet (150% energy requirements). On one occasion the hypercaloric diet contained 5% protein and 25% fat (low protein-high fat, LP-HF), on the other occasion it contained 20% protein and 10% fat (high protein-low fat, HP-LF). IHCL and IMCL concentrations (magnetic resonance spectroscopy) and energy expenditure (indirect calorimetry) were measured after WM, and again after HP-LF/LP-HF. IHCL increased from 25.0 ± 3.6 after WM to 147.1 ± 26.9 mmol/kg wet weight (ww) after LP-HF and from 30.3 ± 7.7 to 57.8 ± 14.8 after HP-LF (two-way ANOVA with interaction: p < 0.001 overfeeding x protein/fat content). IMCL increased from 7.1 ± 0.6 to 8.8 ± 0.7 mmol/kg ww after LP-HF and from 6.2 ± 0.6 to 6.9 ± 0.6 after HP-LF, (p < 0.002). These results indicate that liver and muscle fat deposition is enhanced when sucrose overfeeding is associated with a low protein, high fat diet compared to a high protein, low fat diet. MDPI 2019-01-21 /pmc/articles/PMC6357079/ /pubmed/30669704 http://dx.doi.org/10.3390/nu11010209 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Surowska, Anna
Jegatheesan, Prasanthi
Campos, Vanessa
Marques, Anne-Sophie
Egli, Léonie
Cros, Jérémy
Rosset, Robin
Lecoultre, Virgile
Kreis, Roland
Boesch, Chris
Pouymayou, Bertrand
Schneiter, Philippe
Tappy, Luc
Effects of Dietary Protein and Fat Content on Intrahepatocellular and Intramyocellular Lipids during a 6-Day Hypercaloric, High Sucrose Diet: A Randomized Controlled Trial in Normal Weight Healthy Subjects
title Effects of Dietary Protein and Fat Content on Intrahepatocellular and Intramyocellular Lipids during a 6-Day Hypercaloric, High Sucrose Diet: A Randomized Controlled Trial in Normal Weight Healthy Subjects
title_full Effects of Dietary Protein and Fat Content on Intrahepatocellular and Intramyocellular Lipids during a 6-Day Hypercaloric, High Sucrose Diet: A Randomized Controlled Trial in Normal Weight Healthy Subjects
title_fullStr Effects of Dietary Protein and Fat Content on Intrahepatocellular and Intramyocellular Lipids during a 6-Day Hypercaloric, High Sucrose Diet: A Randomized Controlled Trial in Normal Weight Healthy Subjects
title_full_unstemmed Effects of Dietary Protein and Fat Content on Intrahepatocellular and Intramyocellular Lipids during a 6-Day Hypercaloric, High Sucrose Diet: A Randomized Controlled Trial in Normal Weight Healthy Subjects
title_short Effects of Dietary Protein and Fat Content on Intrahepatocellular and Intramyocellular Lipids during a 6-Day Hypercaloric, High Sucrose Diet: A Randomized Controlled Trial in Normal Weight Healthy Subjects
title_sort effects of dietary protein and fat content on intrahepatocellular and intramyocellular lipids during a 6-day hypercaloric, high sucrose diet: a randomized controlled trial in normal weight healthy subjects
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6357079/
https://www.ncbi.nlm.nih.gov/pubmed/30669704
http://dx.doi.org/10.3390/nu11010209
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