Development of a Bicistronic Vector for the Expression of a CRISPR/Cas9-mCherry System in Fish Cell Lines

The clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9 (CRISPR/Cas9) system has been widely used in animals as an efficient genome editing tool. In fish cells, the technique has been difficult to implement due to the lack of proper vectors that use active promoters...

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Detalles Bibliográficos
Autores principales: Escobar-Aguirre, Sebastian, Arancibia, Duxan, Escorza, Amanda, Bravo, Cristián, Andrés, María Estela, Zamorano, Pedro, Martínez, Víctor
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6357165/
https://www.ncbi.nlm.nih.gov/pubmed/30669572
http://dx.doi.org/10.3390/cells8010075

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6357165/
https://www.ncbi.nlm.nih.gov/pubmed/30669572
http://dx.doi.org/10.3390/cells8010075

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