Cargando…

Subculturing cells have no effect on CRISPR/Cas9‐mediated cleavage of UL30 gene in pseudorabies virus

CRISPR/Cas9‐mediated genome editing can inhibit virus infection by targeting the conserved regions of the viral genomic DNA. Unexpectedly, we found previously that pseudorabies virus (PRV) could escape from CRISPR/Cas9‐mediated inhibition. In order to elucidate whether the escape of PRV from Cas9‐me...

Descripción completa

Detalles Bibliográficos
Autores principales:	Ren, Lin‐zhu, Peng, Zhi‐yuan, Ouyang, Ting, Liu, Xiao‐hui, Chen, Xin‐rong, Ye, Li, Fan, Jun‐wen, Ouyang, Hong‐sheng, Pang, Da‐xin, Bai, Jie‐ying
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	John Wiley and Sons Inc. 2018
Materias:	Short Communication
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6357676/ https://www.ncbi.nlm.nih.gov/pubmed/30891550 http://dx.doi.org/10.1002/ame2.12006

Ejemplares similares

CRISPR/Cas9-Constructed Pseudorabies Virus Mutants Reveal the Importance of UL13 in Alphaherpesvirus Escape from Genome Silencing
por: Van Cleemput, Jolien, et al.
Publicado: (2021)

Genome editing of pseudorabies virus in the CRISPR/Cas9 era: a mini-review
por: Wang, Hai-Ming, et al.
Publicado: (2023)

CRISPR/Cas9-based generation of a recombinant double-reporter pseudorabies virus and its characterization in vitro and in vivo
por: Fu, Peng-Fei, et al.
Publicado: (2021)

Characterization of Nucleocytoplasmic Shuttling of Pseudorabies Virus Protein UL46
por: Xu, Jing-jing, et al.
Publicado: (2020)

Programmable RNA recognition and cleavage by CRISPR/Cas9
por: O’Connell, Mitchell R., et al.
Publicado: (2014)

A systematic mapping study on machine learning techniques for the prediction of CRISPR/Cas9 sgRNA target cleavage
por: Dimauro, Giovanni, et al.
Publicado: (2022)

Conformational control of DNA target cleavage by CRISPR–Cas9
por: Sternberg, Samuel H., et al.
Publicado: (2015)

Nucleosomes inhibit target cleavage by CRISPR-Cas9 in vivo
por: Yarrington, Robert M., et al.
Publicado: (2018)

A cleavage-based surrogate reporter for the evaluation of CRISPR–Cas9 cleavage efficiency
por: Jung, Soo Bin, et al.
Publicado: (2021)

CRISPR/Cas9 Mutagenesis of UL21 in Multiple Strains of Herpes Simplex Virus Reveals Differential Requirements for pUL21 in Viral Replication
por: Finnen, Renée L., et al.
Publicado: (2018)

CRISPR/Cas9 DNA cleavage at SNP-derived PAM enables both in vitro and in vivo KRT12 mutation-specific targeting
por: Courtney, D G, et al.
Publicado: (2016)

Generation and characterization of UL41 null pseudorabies virus variant in vitro and in vivo
por: Ye, Chao, et al.
Publicado: (2018)

Sequence features associated with the cleavage efficiency of CRISPR/Cas9 system
por: Liu, Xiaoxi, et al.
Publicado: (2016)

Structural insights into DNA cleavage activation of CRISPR-Cas9 system
por: Huai, Cong, et al.
Publicado: (2017)

Developing oncolytic Herpes simplex virus type 1 through UL39 knockout by CRISPR-Cas9
por: Ebrahimi, Saeedeh, et al.
Publicado: (2020)

Highly Efficient CRISPR/Cas9-Mediated Homologous Recombination Promotes the Rapid Generation of Bacterial Artificial Chromosomes of Pseudorabies Virus
por: Guo, Jin-Chao, et al.
Publicado: (2016)

Mechanistic insights into the R-loop formation and cleavage in CRISPR-Cas12i1
por: Zhang, Bo, et al.
Publicado: (2021)

Subculture : the meaning of style
por: Hebdige, Dick
Publicado: (1991)

CRISPR/Cas9 editing of APP C-terminus attenuates β-cleavage and promotes α-cleavage
por: Sun, Jichao, et al.
Publicado: (2019)

Pseudorabies virus can be classified into five genotypes using partial sequences of UL44
por: Fonseca Jr., A. A., et al.
Publicado: (2012)

Recombinant Pseudorabies Virus (PRV) Expressing Firefly Luciferase Effectively Screened for CRISPR/Cas9 Single Guide RNAs and Antiviral Compounds
por: Tang, Yan-Dong, et al.
Publicado: (2016)

Structural insights into target DNA recognition and cleavage by the CRISPR-Cas12c1 system
por: Zhang, Bo, et al.
Publicado: (2022)

A conformational checkpoint between DNA binding and cleavage by CRISPR-Cas9
por: Dagdas, Yavuz S., et al.
Publicado: (2017)

Frequent Aneuploidy in Primary Human T Cells after CRISPR-Cas9 cleavage
por: Nahmad, A.D., et al.
Publicado: (2022)

PINCER: improved CRISPR/Cas9 screening by efficient cleavage at conserved residues
por: Veeneman, Brendan, et al.
Publicado: (2020)

Element coding based accurate evaluation of CRISPR/Cas9 initial cleavage
por: Hu, Jianyu, et al.
Publicado: (2021)

Machine learning in the estimation of CRISPR-Cas9 cleavage sites for plant system
por: Das, Jutan, et al.
Publicado: (2023)

Minimal genome-wide human CRISPR-Cas9 library
por: Gonçalves, Emanuel, et al.
Publicado: (2021)

Editor's cut: DNA cleavage by CRISPR RNA-guided nucleases Cas9 and Cas12a
por: Swartjes, Thomas, et al.
Publicado: (2020)

Efficient Generation of Myostatin Mutations in Pigs Using the CRISPR/Cas9 System
por: Wang, Kankan, et al.
Publicado: (2015)

The Pseudorabies Virus DNA Polymerase Accessory Subunit UL42 Directs Nuclear Transport of the Holoenzyme
por: Wang, Yi-Ping, et al.
Publicado: (2016)

Construction of BHV-1 UL41 Defective Virus Using the CRISPR/Cas9 System and Analysis of Viral Replication Properties
por: Dai, Haiyue, et al.
Publicado: (2022)

CRISPR/Cas9 cleavage of viral DNA efficiently suppresses hepatitis B virus
por: Ramanan, Vyas, et al.
Publicado: (2015)

Biased and Unbiased Methods for the Detection of Off-Target Cleavage by CRISPR/Cas9: An Overview
por: Martin, Francisco, et al.
Publicado: (2016)

BoostMEC: predicting CRISPR-Cas9 cleavage efficiency through boosting models
por: Zarate, Oscar A., et al.
Publicado: (2022)

In Vitro Pre-validation of Gene Editing by CRISPR/Cas9 Ribonucleoprotein
por: Mehravar, Maryam, et al.
Publicado: (2019)

piCRISPR: Physically informed deep learning models for CRISPR/Cas9 off-target cleavage prediction
por: Störtz, Florian, et al.
Publicado: (2023)

Increasing on-target cleavage efficiency for CRISPR/Cas9-induced large fragment deletion in Myxococcus xanthus
por: Yang, Ying-jie, et al.
Publicado: (2017)

Nanoparticle Delivery of CRISPR/Cas9 for Genome Editing
por: Duan, Li, et al.
Publicado: (2021)

How subcultures emerge
por: Tureček, Petr, et al.
Publicado: (2023)

Cannot write session to /tmp/vufind_sessions/sess_tj2dk1cghpecphn48f1glpvhc8