Complexes of Pro-Apoptotic siRNAs and Carbosilane Dendrimers: Formation and Effect on Cancer Cells

This paper examines the complexation of anti-cancer small interfering RNAs (siRNAs) by cationic carbosilane dendrimers, and the interaction of the formed complexes with HeLa and HL-60 cancer cells. Stepwise formation of the complexes accompanied by the evolution of their properties has been observed...

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Autores principales: Krasheninina, Olga A., Apartsin, Evgeny K., Fuentes, Elena, Szulc, Aleksandra, Ionov, Maksim, Venyaminova, Alya G., Shcharbin, Dzmitry, de la Mata, F. Javier, Bryszewska, Maria, Gόmez, Rafael
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6359069/
https://www.ncbi.nlm.nih.gov/pubmed/30634643
http://dx.doi.org/10.3390/pharmaceutics11010025
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author Krasheninina, Olga A.
Apartsin, Evgeny K.
Fuentes, Elena
Szulc, Aleksandra
Ionov, Maksim
Venyaminova, Alya G.
Shcharbin, Dzmitry
de la Mata, F. Javier
Bryszewska, Maria
Gόmez, Rafael
author_facet Krasheninina, Olga A.
Apartsin, Evgeny K.
Fuentes, Elena
Szulc, Aleksandra
Ionov, Maksim
Venyaminova, Alya G.
Shcharbin, Dzmitry
de la Mata, F. Javier
Bryszewska, Maria
Gόmez, Rafael
author_sort Krasheninina, Olga A.
collection PubMed
description This paper examines the complexation of anti-cancer small interfering RNAs (siRNAs) by cationic carbosilane dendrimers, and the interaction of the formed complexes with HeLa and HL-60 cancer cells. Stepwise formation of the complexes accompanied by the evolution of their properties has been observed through the increase of the charge ratio (dendrimer/siRNA). The complexes decrease the viability of both “easy-to-transfect” cells (HeLa) and “hard-to transfect” ones (HL-60), indicating a high potential of the cationic carbosilane dendrimers for siRNA delivery into tumor cells.
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spelling pubmed-63590692019-02-14 Complexes of Pro-Apoptotic siRNAs and Carbosilane Dendrimers: Formation and Effect on Cancer Cells Krasheninina, Olga A. Apartsin, Evgeny K. Fuentes, Elena Szulc, Aleksandra Ionov, Maksim Venyaminova, Alya G. Shcharbin, Dzmitry de la Mata, F. Javier Bryszewska, Maria Gόmez, Rafael Pharmaceutics Article This paper examines the complexation of anti-cancer small interfering RNAs (siRNAs) by cationic carbosilane dendrimers, and the interaction of the formed complexes with HeLa and HL-60 cancer cells. Stepwise formation of the complexes accompanied by the evolution of their properties has been observed through the increase of the charge ratio (dendrimer/siRNA). The complexes decrease the viability of both “easy-to-transfect” cells (HeLa) and “hard-to transfect” ones (HL-60), indicating a high potential of the cationic carbosilane dendrimers for siRNA delivery into tumor cells. MDPI 2019-01-10 /pmc/articles/PMC6359069/ /pubmed/30634643 http://dx.doi.org/10.3390/pharmaceutics11010025 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Krasheninina, Olga A.
Apartsin, Evgeny K.
Fuentes, Elena
Szulc, Aleksandra
Ionov, Maksim
Venyaminova, Alya G.
Shcharbin, Dzmitry
de la Mata, F. Javier
Bryszewska, Maria
Gόmez, Rafael
Complexes of Pro-Apoptotic siRNAs and Carbosilane Dendrimers: Formation and Effect on Cancer Cells
title Complexes of Pro-Apoptotic siRNAs and Carbosilane Dendrimers: Formation and Effect on Cancer Cells
title_full Complexes of Pro-Apoptotic siRNAs and Carbosilane Dendrimers: Formation and Effect on Cancer Cells
title_fullStr Complexes of Pro-Apoptotic siRNAs and Carbosilane Dendrimers: Formation and Effect on Cancer Cells
title_full_unstemmed Complexes of Pro-Apoptotic siRNAs and Carbosilane Dendrimers: Formation and Effect on Cancer Cells
title_short Complexes of Pro-Apoptotic siRNAs and Carbosilane Dendrimers: Formation and Effect on Cancer Cells
title_sort complexes of pro-apoptotic sirnas and carbosilane dendrimers: formation and effect on cancer cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6359069/
https://www.ncbi.nlm.nih.gov/pubmed/30634643
http://dx.doi.org/10.3390/pharmaceutics11010025
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