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Imperatorin Suppresses Anaphylactic Reaction and IgE-Mediated Allergic Responses by Inhibiting Multiple Steps of FceRI Signaling in Mast Cells: IMP Alleviates Allergic Responses in PCA

This study is to investigate the effects of imperatorin (IMP) on allergic responses mediated by mast cells, both in vitro and in vivo. Passive cutaneous anaphylaxis (PCA) model was established. Histological detection was performed to assess the ear histology. ELISA and Western blot analysis were use...

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Autores principales: Xian, Zhemin, Jin, Guangyu, Li, Hongmei, Jiang, Jingzhi, Wang, Chongyang, Zhu, Lianhua, Jin, Zhehu, Li, Liangchang, Piao, Hongmei, Zheng, Mingyu, Yan, Guanghai
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6360561/
https://www.ncbi.nlm.nih.gov/pubmed/30800677
http://dx.doi.org/10.1155/2019/7823761
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author Xian, Zhemin
Jin, Guangyu
Li, Hongmei
Jiang, Jingzhi
Wang, Chongyang
Zhu, Lianhua
Jin, Zhehu
Li, Liangchang
Piao, Hongmei
Zheng, Mingyu
Yan, Guanghai
author_facet Xian, Zhemin
Jin, Guangyu
Li, Hongmei
Jiang, Jingzhi
Wang, Chongyang
Zhu, Lianhua
Jin, Zhehu
Li, Liangchang
Piao, Hongmei
Zheng, Mingyu
Yan, Guanghai
author_sort Xian, Zhemin
collection PubMed
description This study is to investigate the effects of imperatorin (IMP) on allergic responses mediated by mast cells, both in vitro and in vivo. Passive cutaneous anaphylaxis (PCA) model was established. Histological detection was performed to assess the ear histology. ELISA and Western blot analysis were used to detect the levels of corresponding cytokines and signalling pathway proteins. IMP decreased the leakage of Evans blue and the ear thickness in the PCA models, in a dose-dependent manner, and alleviated the degranulation of mast cells. Moreover, IMP reduced the expression of TNF-α, IL-4, IL-1β, IL-8, and IL-13. Furthermore, IMP inhibited the phosphorylation levels of Syk, Lyn, PLC-γ1, and Gab2, as well as the downstream MAPK, PI3K/AKT, and NF-κB signaling pathways. In addition, IMP inhibited the mast cell-mediated allergic responses through the Nrf2/HO-1 pathway. IMP attenuates the allergic responses through inhibiting the degranulation and decreasing the expression levels of proinflammatory cytokines in the mast cells, involving the PI3K/Akt, MAPK, NF-κB, and Nrf2/HO-1 pathways.
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spelling pubmed-63605612019-02-24 Imperatorin Suppresses Anaphylactic Reaction and IgE-Mediated Allergic Responses by Inhibiting Multiple Steps of FceRI Signaling in Mast Cells: IMP Alleviates Allergic Responses in PCA Xian, Zhemin Jin, Guangyu Li, Hongmei Jiang, Jingzhi Wang, Chongyang Zhu, Lianhua Jin, Zhehu Li, Liangchang Piao, Hongmei Zheng, Mingyu Yan, Guanghai Biomed Res Int Research Article This study is to investigate the effects of imperatorin (IMP) on allergic responses mediated by mast cells, both in vitro and in vivo. Passive cutaneous anaphylaxis (PCA) model was established. Histological detection was performed to assess the ear histology. ELISA and Western blot analysis were used to detect the levels of corresponding cytokines and signalling pathway proteins. IMP decreased the leakage of Evans blue and the ear thickness in the PCA models, in a dose-dependent manner, and alleviated the degranulation of mast cells. Moreover, IMP reduced the expression of TNF-α, IL-4, IL-1β, IL-8, and IL-13. Furthermore, IMP inhibited the phosphorylation levels of Syk, Lyn, PLC-γ1, and Gab2, as well as the downstream MAPK, PI3K/AKT, and NF-κB signaling pathways. In addition, IMP inhibited the mast cell-mediated allergic responses through the Nrf2/HO-1 pathway. IMP attenuates the allergic responses through inhibiting the degranulation and decreasing the expression levels of proinflammatory cytokines in the mast cells, involving the PI3K/Akt, MAPK, NF-κB, and Nrf2/HO-1 pathways. Hindawi 2019-01-20 /pmc/articles/PMC6360561/ /pubmed/30800677 http://dx.doi.org/10.1155/2019/7823761 Text en Copyright © 2019 Zhemin Xian et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Xian, Zhemin
Jin, Guangyu
Li, Hongmei
Jiang, Jingzhi
Wang, Chongyang
Zhu, Lianhua
Jin, Zhehu
Li, Liangchang
Piao, Hongmei
Zheng, Mingyu
Yan, Guanghai
Imperatorin Suppresses Anaphylactic Reaction and IgE-Mediated Allergic Responses by Inhibiting Multiple Steps of FceRI Signaling in Mast Cells: IMP Alleviates Allergic Responses in PCA
title Imperatorin Suppresses Anaphylactic Reaction and IgE-Mediated Allergic Responses by Inhibiting Multiple Steps of FceRI Signaling in Mast Cells: IMP Alleviates Allergic Responses in PCA
title_full Imperatorin Suppresses Anaphylactic Reaction and IgE-Mediated Allergic Responses by Inhibiting Multiple Steps of FceRI Signaling in Mast Cells: IMP Alleviates Allergic Responses in PCA
title_fullStr Imperatorin Suppresses Anaphylactic Reaction and IgE-Mediated Allergic Responses by Inhibiting Multiple Steps of FceRI Signaling in Mast Cells: IMP Alleviates Allergic Responses in PCA
title_full_unstemmed Imperatorin Suppresses Anaphylactic Reaction and IgE-Mediated Allergic Responses by Inhibiting Multiple Steps of FceRI Signaling in Mast Cells: IMP Alleviates Allergic Responses in PCA
title_short Imperatorin Suppresses Anaphylactic Reaction and IgE-Mediated Allergic Responses by Inhibiting Multiple Steps of FceRI Signaling in Mast Cells: IMP Alleviates Allergic Responses in PCA
title_sort imperatorin suppresses anaphylactic reaction and ige-mediated allergic responses by inhibiting multiple steps of fceri signaling in mast cells: imp alleviates allergic responses in pca
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6360561/
https://www.ncbi.nlm.nih.gov/pubmed/30800677
http://dx.doi.org/10.1155/2019/7823761
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