Enhanced mammalian genome editing by new Cas12a orthologs with optimized crRNA scaffolds

CRISPR-Cas12a/Cpf1, a single RNA-guided endonuclease system, provides a promising tool for genome engineering. However, only three Cas12a orthologs have been employed for mammalian genome editing, and the editing efficiency as well as targeting coverage still requires improvements. Here, we harness...

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Detalles Bibliográficos
Autores principales: Teng, Fei, Li, Jing, Cui, Tongtong, Xu, Kai, Guo, Lu, Gao, Qingqin, Feng, Guihai, Chen, Chuanyuan, Han, Dali, Zhou, Qi, Li, Wei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2019
Materias:
Short Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6362571/
https://www.ncbi.nlm.nih.gov/pubmed/30717767
http://dx.doi.org/10.1186/s13059-019-1620-8

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6362571/
https://www.ncbi.nlm.nih.gov/pubmed/30717767
http://dx.doi.org/10.1186/s13059-019-1620-8

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