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Collagen reorganization in cartilage under strain probed by polarization sensitive second harmonic generation microscopy

Type II collagen fibril diameters in cartilage are beneath the diffraction limit of optical microscopy, which makes the assessment of collagen organization very challenging. In this work we use polarization sensitive second harmonic generation (P-SHG) imaging to map collagen organization in articula...

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Autores principales: Mansfield, Jessica C., Mandalia, Vipul, Toms, Andrew, Winlove, C. Peter, Brasselet, Sophie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6364654/
https://www.ncbi.nlm.nih.gov/pubmed/30958161
http://dx.doi.org/10.1098/rsif.2018.0611
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author Mansfield, Jessica C.
Mandalia, Vipul
Toms, Andrew
Winlove, C. Peter
Brasselet, Sophie
author_facet Mansfield, Jessica C.
Mandalia, Vipul
Toms, Andrew
Winlove, C. Peter
Brasselet, Sophie
author_sort Mansfield, Jessica C.
collection PubMed
description Type II collagen fibril diameters in cartilage are beneath the diffraction limit of optical microscopy, which makes the assessment of collagen organization very challenging. In this work we use polarization sensitive second harmonic generation (P-SHG) imaging to map collagen organization in articular cartilage, addressing in particular its behaviour under strain and changes which occur in osteoarthritis. P-SHG yields two parameters, molecular order and orientation, which provide measures of the degree of organization both at the molecular scale (below the diffraction limit) and above a few hundred nanometres (at the image pixel size). P-SHG clearly demonstrates the zonal collagen architecture and reveals differences in the structure of the fibrils around chondrocytes. P-SHG also reveals sub-micron scale fibril re-organization in cartilage strips exposed to tensile loading, with an increase in local organization in the superficial zone which weakly correlates with tensile modulus. Finally, P-SHG is used to investigate osteoarthritic cartilage from total knee replacement surgery, and reveals widespread heterogeneity across samples both microscale fibril orientations and their sub-micron organization. By addressing collagen fibril structure on scales intermediate between conventional light and electron microscopy, this study provides new insights into collagen micromechanics and mechanisms of degradation.
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spelling pubmed-63646542019-02-13 Collagen reorganization in cartilage under strain probed by polarization sensitive second harmonic generation microscopy Mansfield, Jessica C. Mandalia, Vipul Toms, Andrew Winlove, C. Peter Brasselet, Sophie J R Soc Interface Life Sciences–Physics interface Type II collagen fibril diameters in cartilage are beneath the diffraction limit of optical microscopy, which makes the assessment of collagen organization very challenging. In this work we use polarization sensitive second harmonic generation (P-SHG) imaging to map collagen organization in articular cartilage, addressing in particular its behaviour under strain and changes which occur in osteoarthritis. P-SHG yields two parameters, molecular order and orientation, which provide measures of the degree of organization both at the molecular scale (below the diffraction limit) and above a few hundred nanometres (at the image pixel size). P-SHG clearly demonstrates the zonal collagen architecture and reveals differences in the structure of the fibrils around chondrocytes. P-SHG also reveals sub-micron scale fibril re-organization in cartilage strips exposed to tensile loading, with an increase in local organization in the superficial zone which weakly correlates with tensile modulus. Finally, P-SHG is used to investigate osteoarthritic cartilage from total knee replacement surgery, and reveals widespread heterogeneity across samples both microscale fibril orientations and their sub-micron organization. By addressing collagen fibril structure on scales intermediate between conventional light and electron microscopy, this study provides new insights into collagen micromechanics and mechanisms of degradation. The Royal Society 2019-01 2019-01-16 /pmc/articles/PMC6364654/ /pubmed/30958161 http://dx.doi.org/10.1098/rsif.2018.0611 Text en © 2019 The Authors. http://creativecommons.org/licenses/by/4.0/ Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/, which permits unrestricted use, provided the original author and source are credited.
spellingShingle Life Sciences–Physics interface
Mansfield, Jessica C.
Mandalia, Vipul
Toms, Andrew
Winlove, C. Peter
Brasselet, Sophie
Collagen reorganization in cartilage under strain probed by polarization sensitive second harmonic generation microscopy
title Collagen reorganization in cartilage under strain probed by polarization sensitive second harmonic generation microscopy
title_full Collagen reorganization in cartilage under strain probed by polarization sensitive second harmonic generation microscopy
title_fullStr Collagen reorganization in cartilage under strain probed by polarization sensitive second harmonic generation microscopy
title_full_unstemmed Collagen reorganization in cartilage under strain probed by polarization sensitive second harmonic generation microscopy
title_short Collagen reorganization in cartilage under strain probed by polarization sensitive second harmonic generation microscopy
title_sort collagen reorganization in cartilage under strain probed by polarization sensitive second harmonic generation microscopy
topic Life Sciences–Physics interface
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6364654/
https://www.ncbi.nlm.nih.gov/pubmed/30958161
http://dx.doi.org/10.1098/rsif.2018.0611
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