Evaluation of Oral Cavity DNA Extraction Methods on Bacterial and Fungal Microbiota

The objective of this study was to evaluate the most effective method of DNA extraction of oral mouthwash samples for use in microbiome studies that utilize next generation sequencing (NGS). Eight enzymatic and mechanical DNA extraction methods were tested. Extracted DNA was amplified using barcoded...

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Detalles Bibliográficos
Autores principales: Rosenbaum, Jennifer, Usyk, Mykhaylo, Chen, Zigui, Zolnik, Christine P., Jones, Heidi E., Waldron, Levi, Dowd, Jennifer B., Thorpe, Lorna E., Burk, Robert D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6365504/
https://www.ncbi.nlm.nih.gov/pubmed/30728424
http://dx.doi.org/10.1038/s41598-018-38049-6

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6365504/
https://www.ncbi.nlm.nih.gov/pubmed/30728424
http://dx.doi.org/10.1038/s41598-018-38049-6

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