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Evaluation of Oral Cavity DNA Extraction Methods on Bacterial and Fungal Microbiota
The objective of this study was to evaluate the most effective method of DNA extraction of oral mouthwash samples for use in microbiome studies that utilize next generation sequencing (NGS). Eight enzymatic and mechanical DNA extraction methods were tested. Extracted DNA was amplified using barcoded...
Autores principales: | Rosenbaum, Jennifer, Usyk, Mykhaylo, Chen, Zigui, Zolnik, Christine P., Jones, Heidi E., Waldron, Levi, Dowd, Jennifer B., Thorpe, Lorna E., Burk, Robert D. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6365504/ https://www.ncbi.nlm.nih.gov/pubmed/30728424 http://dx.doi.org/10.1038/s41598-018-38049-6 |
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