Allergy to carminic acid: in vitro evidence of involvement of protein-binding hapten

We previously described a rare case of anaphylaxis presumably induced by carminic acid in cochineal dye used as a food additive. In this study, highly pure carminic acid was added to an albumin-containing buffer at various concentrations, followed by serial dilution. Varying the mixing ratio of carm...

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Autores principales: Osumi, Mika, Yamaguchi, Masao, Sugimoto, Naoya, Suzukawa, Maho, Arai, Hidenori, Akiyama, Hiroshi, Nagase, Hiroyuki, Ohta, Ken
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Asia Pacific Association of Allergy, Asthma and Clinical Immunology 2019
Materias:
Letter to the Editor
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6365656/
https://www.ncbi.nlm.nih.gov/pubmed/30740350
http://dx.doi.org/10.5415/apallergy.2019.9.e2
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author Osumi, Mika
Yamaguchi, Masao
Sugimoto, Naoya
Suzukawa, Maho
Arai, Hidenori
Akiyama, Hiroshi
Nagase, Hiroyuki
Ohta, Ken
author_facet Osumi, Mika
Yamaguchi, Masao
Sugimoto, Naoya
Suzukawa, Maho
Arai, Hidenori
Akiyama, Hiroshi
Nagase, Hiroyuki
Ohta, Ken
author_sort Osumi, Mika
collection PubMed
description We previously described a rare case of anaphylaxis presumably induced by carminic acid in cochineal dye used as a food additive. In this study, highly pure carminic acid was added to an albumin-containing buffer at various concentrations, followed by serial dilution. Varying the mixing ratio of carminic acid and albumin affected the extent of histamine release from passively sensitized basophils. Similar basophil histamine release occurred with carminic acid-globulin solutions. These results provide experimental evidence indicating that basophil activation is dependent on hapten (carminic acid) and carrier (protein) interaction.
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spelling pubmed-63656562019-02-08 Allergy to carminic acid: in vitro evidence of involvement of protein-binding hapten Osumi, Mika Yamaguchi, Masao Sugimoto, Naoya Suzukawa, Maho Arai, Hidenori Akiyama, Hiroshi Nagase, Hiroyuki Ohta, Ken Asia Pac Allergy Letter to the Editor We previously described a rare case of anaphylaxis presumably induced by carminic acid in cochineal dye used as a food additive. In this study, highly pure carminic acid was added to an albumin-containing buffer at various concentrations, followed by serial dilution. Varying the mixing ratio of carminic acid and albumin affected the extent of histamine release from passively sensitized basophils. Similar basophil histamine release occurred with carminic acid-globulin solutions. These results provide experimental evidence indicating that basophil activation is dependent on hapten (carminic acid) and carrier (protein) interaction. Asia Pacific Association of Allergy, Asthma and Clinical Immunology 2019-01-14 /pmc/articles/PMC6365656/ /pubmed/30740350 http://dx.doi.org/10.5415/apallergy.2019.9.e2 Text en Copyright © 2019. Asia Pacific Association of Allergy, Asthma and Clinical Immunology. https://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (https://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Letter to the Editor
Osumi, Mika
Yamaguchi, Masao
Sugimoto, Naoya
Suzukawa, Maho
Arai, Hidenori
Akiyama, Hiroshi
Nagase, Hiroyuki
Ohta, Ken
Allergy to carminic acid: in vitro evidence of involvement of protein-binding hapten
title Allergy to carminic acid: in vitro evidence of involvement of protein-binding hapten
title_full Allergy to carminic acid: in vitro evidence of involvement of protein-binding hapten
title_fullStr Allergy to carminic acid: in vitro evidence of involvement of protein-binding hapten
title_full_unstemmed Allergy to carminic acid: in vitro evidence of involvement of protein-binding hapten
title_short Allergy to carminic acid: in vitro evidence of involvement of protein-binding hapten
title_sort allergy to carminic acid: in vitro evidence of involvement of protein-binding hapten
topic Letter to the Editor
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6365656/
https://www.ncbi.nlm.nih.gov/pubmed/30740350
http://dx.doi.org/10.5415/apallergy.2019.9.e2
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