An Evolved Methanomethylophilus alvus Pyrrolysyl-tRNA Synthetase/tRNA Pair Is Highly Active and Orthogonal in Mammalian Cells

[Image: see text] We recently characterized a new class of pyrrolysyl-tRNA synthetase (PylRS)/(Pyl)tRNA pairs from Methanomassiliicocales that are active and orthogonal in Escherichia coli. The aminoacyl-tRNA synthetases (aaRSs) of these pairs lack the N-terminal domain that is essential for tRNA re...

Descripción completa

Detalles Bibliográficos
Autores principales: Beránek, Václav, Willis, Julian C. W., Chin, Jason W.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2018
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6365905/
https://www.ncbi.nlm.nih.gov/pubmed/30260626
http://dx.doi.org/10.1021/acs.biochem.8b00808

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6365905/
https://www.ncbi.nlm.nih.gov/pubmed/30260626
http://dx.doi.org/10.1021/acs.biochem.8b00808

Ejemplares similares