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The YoaW signal peptide directs efficient secretion of different heterologous proteins fused to a StrepII-SUMO tag in Bacillus subtilis
BACKGROUND: Heterologous gene expression is well established for various prokaryotic model systems. However, low yield, incorrect folding and instability still impede the production of soluble, bioactive proteins. To improve protein production with the Gram-positive host Bacillus subtilis, a secreto...
Autores principales: | Heinrich, Janine, Drewniok, Chris, Neugebauer, Eva, Kellner, Harald, Wiegert, Thomas |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6366066/ https://www.ncbi.nlm.nih.gov/pubmed/30732606 http://dx.doi.org/10.1186/s12934-019-1078-0 |
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