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In silico re-assessment of a diagnostic RT-qPCR assay for universal detection of Influenza A viruses

The ongoing evolution of microbial pathogens represents a significant issue in diagnostic PCR/qPCR. Many assays are burdened with false negativity due to mispriming and/or probe-binding failures. Therefore, PCR/qPCR assays used in the laboratory should be periodically re-assessed in silico on public...

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Detalles Bibliográficos
Autores principales: Nagy, Alexander, Jiřinec, Tomáš, Jiřincová, Helena, Černíková, Lenka, Havlíčková, Martina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6367508/
https://www.ncbi.nlm.nih.gov/pubmed/30733500
http://dx.doi.org/10.1038/s41598-018-37869-w