RppH can faithfully replace TAP to allow cloning of 5′-triphosphate carrying small RNAs

RNA interference was first described in the nematode Caenorhabditis elegans. Ever since, several new endogenous small RNA pathways have been described and characterized to different degrees. The very prominent secondary small interfering RNAs, also called 22G-RNAs, bear a 5′ triphosphate group after...

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Detalles Bibliográficos
Autores principales: Almeida, Miguel Vasconcelos, de Jesus Domingues, António Miguel, Lukas, Hanna, Mendez-Lago, Maria, Ketting, René F.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2019
Materias:
Biochemistry, Genetics and Molecular Biology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6369235/
https://www.ncbi.nlm.nih.gov/pubmed/30788220
http://dx.doi.org/10.1016/j.mex.2019.01.009

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6369235/
https://www.ncbi.nlm.nih.gov/pubmed/30788220
http://dx.doi.org/10.1016/j.mex.2019.01.009

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