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Microbiota profiling with long amplicons using Nanopore sequencing: full-length 16S rRNA gene and the 16S-ITS-23S of the  rrn operon

Background: Profiling the microbiome of low-biomass samples is challenging for metagenomics since these samples are prone to contain DNA from other sources (e.g. host or environment). The usual approach is sequencing short regions of the 16S rRNA gene, which fails to assign taxonomy to genus and spe...

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Detalles Bibliográficos
Autores principales: Cuscó, Anna, Catozzi, Carlotta, Viñes, Joaquim, Sanchez, Armand, Francino, Olga
Formato: Online Artículo Texto
Lenguaje:English
Publicado: F1000 Research Limited 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6372929/
https://www.ncbi.nlm.nih.gov/pubmed/30815250
http://dx.doi.org/10.12688/f1000research.16817.2