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Are These Cardiomyocytes? Protocol Development Reveals Impact of Sample Preparation on the Accuracy of Identifying Cardiomyocytes by Flow Cytometry

Several protocols now support efficient differentiation of human pluripotent stem cells to cardiomyocytes (hPSC-CMs) but these still indicate line-to-line variability. As the number of studies implementing this technology expands, accurate assessment of cell identity is paramount to well-defined stu...

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Detalles Bibliográficos
Autores principales: Waas, Matthew, Weerasekera, Ranjuna, Kropp, Erin M., Romero-Tejeda, Marisol, Poon, Ellen N., Boheler, Kenneth R., Burridge, Paul W., Gundry, Rebekah L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6373208/
https://www.ncbi.nlm.nih.gov/pubmed/30686762
http://dx.doi.org/10.1016/j.stemcr.2018.12.016