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Are These Cardiomyocytes? Protocol Development Reveals Impact of Sample Preparation on the Accuracy of Identifying Cardiomyocytes by Flow Cytometry
Several protocols now support efficient differentiation of human pluripotent stem cells to cardiomyocytes (hPSC-CMs) but these still indicate line-to-line variability. As the number of studies implementing this technology expands, accurate assessment of cell identity is paramount to well-defined stu...
Autores principales: | Waas, Matthew, Weerasekera, Ranjuna, Kropp, Erin M., Romero-Tejeda, Marisol, Poon, Ellen N., Boheler, Kenneth R., Burridge, Paul W., Gundry, Rebekah L. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6373208/ https://www.ncbi.nlm.nih.gov/pubmed/30686762 http://dx.doi.org/10.1016/j.stemcr.2018.12.016 |
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