Nanometric axial resolution of fibronectin assembly units achieved with an efficient reconstruction approach for multi-angle-TIRF microscopy

High resolution imaging of molecules at the cell-substrate interface is required for understanding key biological processes. Here we propose a complete pipeline for multi-angle total internal reflection fluorescence microscopy (MA-TIRF) going from instrument design and calibration procedures to nume...

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Detalles Bibliográficos
Autores principales: Soubies, Emmanuel, Radwanska, Agata, Grall, Dominique, Blanc-Féraud, Laure, Van Obberghen-Schilling, Ellen, Schaub, Sébastien
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6374485/
https://www.ncbi.nlm.nih.gov/pubmed/30760745
http://dx.doi.org/10.1038/s41598-018-36119-3

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6374485/
https://www.ncbi.nlm.nih.gov/pubmed/30760745
http://dx.doi.org/10.1038/s41598-018-36119-3

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