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Genome-Wide Association Mapping of Grain Micronutrients Concentration in Aegilops tauschii

Bread wheat is an important and the most consumed cereal worldwide. However, people with predominantly cereal-based diets are increasingly affected by micronutrient deficiencies, suggesting the need for biofortified wheat varieties. The limited genetic diversity in hexaploid wheat warrants exploring...

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Autores principales: Arora, Sanu, Cheema, Jitender, Poland, Jesse, Uauy, Cristobal, Chhuneja, Parveen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6374599/
https://www.ncbi.nlm.nih.gov/pubmed/30792723
http://dx.doi.org/10.3389/fpls.2019.00054
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author Arora, Sanu
Cheema, Jitender
Poland, Jesse
Uauy, Cristobal
Chhuneja, Parveen
author_facet Arora, Sanu
Cheema, Jitender
Poland, Jesse
Uauy, Cristobal
Chhuneja, Parveen
author_sort Arora, Sanu
collection PubMed
description Bread wheat is an important and the most consumed cereal worldwide. However, people with predominantly cereal-based diets are increasingly affected by micronutrient deficiencies, suggesting the need for biofortified wheat varieties. The limited genetic diversity in hexaploid wheat warrants exploring the wider variation present in wheat wild relatives, among these Aegilops tauschii, the wild progenitor of the bread wheat D genome. In this study, a panel of 167 Ae. tauschii accessions was phenotyped for grain Fe, Zn, Cu, and Mn concentrations for 3 years and was found to have wide variation for these micronutrients. Comparisons between the two genetic subpopulations of Ae. tauschii revealed that lineage 2 had higher mean values for Fe and Cu concentration than lineage 1. To identify potentially new genetic sources for improving grain micronutrient concentration, we performed a genome-wide association study (GWAS) on 114 non-redundant Ae. tauschii accessions using 5,249 genotyping-by-sequencing (GBS) markers. Best linear unbiased predictor (BLUP) values were calculated for all traits across the three growing seasons. A total of 19 SNP marker trait associations (MTAs) were detected for all traits after applying Bonferroni corrected threshold of -log(10)(P-value) ≥ 4.68. These MTAs were found on all seven chromosomes. For grain Fe, Zn, Cu, and Mn concentrations, five, four, three, and seven significant associations were detected, respectively. The associations were linked to the genes encoding transcription factor regulators, transporters, and phytosiderophore synthesis. The results demonstrate the utility of GWAS for understanding the genetic architecture of micronutrient accumulation in Ae. tauschii, and further efforts to validate these loci will aid in using them to diversify the D-genome of hexaploid wheat.
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spelling pubmed-63745992019-02-21 Genome-Wide Association Mapping of Grain Micronutrients Concentration in Aegilops tauschii Arora, Sanu Cheema, Jitender Poland, Jesse Uauy, Cristobal Chhuneja, Parveen Front Plant Sci Plant Science Bread wheat is an important and the most consumed cereal worldwide. However, people with predominantly cereal-based diets are increasingly affected by micronutrient deficiencies, suggesting the need for biofortified wheat varieties. The limited genetic diversity in hexaploid wheat warrants exploring the wider variation present in wheat wild relatives, among these Aegilops tauschii, the wild progenitor of the bread wheat D genome. In this study, a panel of 167 Ae. tauschii accessions was phenotyped for grain Fe, Zn, Cu, and Mn concentrations for 3 years and was found to have wide variation for these micronutrients. Comparisons between the two genetic subpopulations of Ae. tauschii revealed that lineage 2 had higher mean values for Fe and Cu concentration than lineage 1. To identify potentially new genetic sources for improving grain micronutrient concentration, we performed a genome-wide association study (GWAS) on 114 non-redundant Ae. tauschii accessions using 5,249 genotyping-by-sequencing (GBS) markers. Best linear unbiased predictor (BLUP) values were calculated for all traits across the three growing seasons. A total of 19 SNP marker trait associations (MTAs) were detected for all traits after applying Bonferroni corrected threshold of -log(10)(P-value) ≥ 4.68. These MTAs were found on all seven chromosomes. For grain Fe, Zn, Cu, and Mn concentrations, five, four, three, and seven significant associations were detected, respectively. The associations were linked to the genes encoding transcription factor regulators, transporters, and phytosiderophore synthesis. The results demonstrate the utility of GWAS for understanding the genetic architecture of micronutrient accumulation in Ae. tauschii, and further efforts to validate these loci will aid in using them to diversify the D-genome of hexaploid wheat. Frontiers Media S.A. 2019-02-07 /pmc/articles/PMC6374599/ /pubmed/30792723 http://dx.doi.org/10.3389/fpls.2019.00054 Text en Copyright © 2019 Arora, Cheema, Poland, Uauy and Chhuneja. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Arora, Sanu
Cheema, Jitender
Poland, Jesse
Uauy, Cristobal
Chhuneja, Parveen
Genome-Wide Association Mapping of Grain Micronutrients Concentration in Aegilops tauschii
title Genome-Wide Association Mapping of Grain Micronutrients Concentration in Aegilops tauschii
title_full Genome-Wide Association Mapping of Grain Micronutrients Concentration in Aegilops tauschii
title_fullStr Genome-Wide Association Mapping of Grain Micronutrients Concentration in Aegilops tauschii
title_full_unstemmed Genome-Wide Association Mapping of Grain Micronutrients Concentration in Aegilops tauschii
title_short Genome-Wide Association Mapping of Grain Micronutrients Concentration in Aegilops tauschii
title_sort genome-wide association mapping of grain micronutrients concentration in aegilops tauschii
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6374599/
https://www.ncbi.nlm.nih.gov/pubmed/30792723
http://dx.doi.org/10.3389/fpls.2019.00054
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