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Shedding light: The importance of reverse transcription efficiency standards in data interpretation
The RNA-to-cDNA conversion step in transcriptomics experiments is widely recognised as inefficient and variable, casting doubt on the ability to do quantitative transcriptomics analyses. Multiple studies have focused on ways to optimise this process, resulting in contradictory recommendations. Here...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6374950/ https://www.ncbi.nlm.nih.gov/pubmed/30805297 http://dx.doi.org/10.1016/j.bdq.2018.12.002 |