Linear-hairpin variable primer RT-qPCR for MicroRNA

Here, we present a highly specific, sensitive and cost-effective system to quantify microRNA (miRNA) expression based on two-step RT-qPCR with EvaGreen detection chemistry, called linear-hairpin variable primer RT-qPCR. It takes advantage of the novel designed variable primer, which is initially des...

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Detalles Bibliográficos
Autores principales: Lan, Lin, Guo, Qiuping, Nie, Hemin, Zhou, Chang, Cai, Qingyun, Huang, Jin, Meng, Xiangxian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Royal Society of Chemistry 2018
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6375362/
https://www.ncbi.nlm.nih.gov/pubmed/30842860
http://dx.doi.org/10.1039/c8sc04621b

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6375362/
https://www.ncbi.nlm.nih.gov/pubmed/30842860
http://dx.doi.org/10.1039/c8sc04621b

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