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Advances in geometric techniques for analyzing blebbing in chemotaxing Dictyostelium cells

We present a technical platform that allows us to monitor and measure cortex and membrane dynamics during bleb-based chemotaxis. Using D. discoideum cells expressing LifeAct-GFP and crawling under agarose containing RITC-dextran, we were able to simultaneously visualize the actin cortex and the cell...

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Detalles Bibliográficos
Autores principales: Santiago, Zully, Loustau, John, Meretzky, David, Rawal, Devarshi, Brazill, Derrick
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6375592/
https://www.ncbi.nlm.nih.gov/pubmed/30763409
http://dx.doi.org/10.1371/journal.pone.0211975