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Advances in geometric techniques for analyzing blebbing in chemotaxing Dictyostelium cells
We present a technical platform that allows us to monitor and measure cortex and membrane dynamics during bleb-based chemotaxis. Using D. discoideum cells expressing LifeAct-GFP and crawling under agarose containing RITC-dextran, we were able to simultaneously visualize the actin cortex and the cell...
Autores principales: | Santiago, Zully, Loustau, John, Meretzky, David, Rawal, Devarshi, Brazill, Derrick |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6375592/ https://www.ncbi.nlm.nih.gov/pubmed/30763409 http://dx.doi.org/10.1371/journal.pone.0211975 |
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