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Protein–Protein Affinity Determination by Quantitative FRET Quenching
The molecular dissociation constant, K(d), is a well-established parameter to quantitate the affinity of protein-protein or other molecular interactions. Recently, we reported the theoretical basis and experimental procedure for K(d) determination using a quantitative FRET method. Here we report a n...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6375914/ https://www.ncbi.nlm.nih.gov/pubmed/30765720 http://dx.doi.org/10.1038/s41598-018-35535-9 |