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Lambda bacteriophage nanoparticles displaying GP2, a HER2/neu derived peptide, induce prophylactic and therapeutic activities against TUBO tumor model in mice

Generating a protective and long-lasting immune response is the primary goal in the expanding field of immunotherapeutic research. In current study we designed an immunogenic bacteriophage- based vaccine to induce a cytotoxic T lymphocyte activity against a mice tumor model over-expressing HER2/neu....

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Autores principales: Razazan, Atefeh, Nicastro, Jessica, Slavcev, Roderick, Barati, Nastaran, Arab, Atefeh, Mosaffa, Fatemeh, Jaafari, Mahmoud Reza, Behravan, Javad
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6379380/
https://www.ncbi.nlm.nih.gov/pubmed/30778090
http://dx.doi.org/10.1038/s41598-018-38371-z
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author Razazan, Atefeh
Nicastro, Jessica
Slavcev, Roderick
Barati, Nastaran
Arab, Atefeh
Mosaffa, Fatemeh
Jaafari, Mahmoud Reza
Behravan, Javad
author_facet Razazan, Atefeh
Nicastro, Jessica
Slavcev, Roderick
Barati, Nastaran
Arab, Atefeh
Mosaffa, Fatemeh
Jaafari, Mahmoud Reza
Behravan, Javad
author_sort Razazan, Atefeh
collection PubMed
description Generating a protective and long-lasting immune response is the primary goal in the expanding field of immunotherapeutic research. In current study we designed an immunogenic bacteriophage- based vaccine to induce a cytotoxic T lymphocyte activity against a mice tumor model over-expressing HER2/neu. Bacteriophage λ displaying a HER2/neu derived peptide GP2 was constructed and used as an anti-cancer vaccine in a BALB/c mouse xenograft tumor model. The results of our study indicated that phage nanoparticles displaying GP2 as a fused peptide to the gpD phage capsid protein induced a robust CTL response. Furthermore, the chimeric phage nanoparticles protected mice against HER2/neu-positive tumor challenge in both prophylactic and therapeutic settings. In conclusion, we propose that λ phage nanoparticles decorated with GP2 peptide merit further investigation for the development of peptide-based vaccines against HER2/neu overexpressing tumors.
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spelling pubmed-63793802019-02-21 Lambda bacteriophage nanoparticles displaying GP2, a HER2/neu derived peptide, induce prophylactic and therapeutic activities against TUBO tumor model in mice Razazan, Atefeh Nicastro, Jessica Slavcev, Roderick Barati, Nastaran Arab, Atefeh Mosaffa, Fatemeh Jaafari, Mahmoud Reza Behravan, Javad Sci Rep Article Generating a protective and long-lasting immune response is the primary goal in the expanding field of immunotherapeutic research. In current study we designed an immunogenic bacteriophage- based vaccine to induce a cytotoxic T lymphocyte activity against a mice tumor model over-expressing HER2/neu. Bacteriophage λ displaying a HER2/neu derived peptide GP2 was constructed and used as an anti-cancer vaccine in a BALB/c mouse xenograft tumor model. The results of our study indicated that phage nanoparticles displaying GP2 as a fused peptide to the gpD phage capsid protein induced a robust CTL response. Furthermore, the chimeric phage nanoparticles protected mice against HER2/neu-positive tumor challenge in both prophylactic and therapeutic settings. In conclusion, we propose that λ phage nanoparticles decorated with GP2 peptide merit further investigation for the development of peptide-based vaccines against HER2/neu overexpressing tumors. Nature Publishing Group UK 2019-02-18 /pmc/articles/PMC6379380/ /pubmed/30778090 http://dx.doi.org/10.1038/s41598-018-38371-z Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Razazan, Atefeh
Nicastro, Jessica
Slavcev, Roderick
Barati, Nastaran
Arab, Atefeh
Mosaffa, Fatemeh
Jaafari, Mahmoud Reza
Behravan, Javad
Lambda bacteriophage nanoparticles displaying GP2, a HER2/neu derived peptide, induce prophylactic and therapeutic activities against TUBO tumor model in mice
title Lambda bacteriophage nanoparticles displaying GP2, a HER2/neu derived peptide, induce prophylactic and therapeutic activities against TUBO tumor model in mice
title_full Lambda bacteriophage nanoparticles displaying GP2, a HER2/neu derived peptide, induce prophylactic and therapeutic activities against TUBO tumor model in mice
title_fullStr Lambda bacteriophage nanoparticles displaying GP2, a HER2/neu derived peptide, induce prophylactic and therapeutic activities against TUBO tumor model in mice
title_full_unstemmed Lambda bacteriophage nanoparticles displaying GP2, a HER2/neu derived peptide, induce prophylactic and therapeutic activities against TUBO tumor model in mice
title_short Lambda bacteriophage nanoparticles displaying GP2, a HER2/neu derived peptide, induce prophylactic and therapeutic activities against TUBO tumor model in mice
title_sort lambda bacteriophage nanoparticles displaying gp2, a her2/neu derived peptide, induce prophylactic and therapeutic activities against tubo tumor model in mice
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6379380/
https://www.ncbi.nlm.nih.gov/pubmed/30778090
http://dx.doi.org/10.1038/s41598-018-38371-z
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