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scFTD-seq: freeze-thaw lysis based, portable approach toward highly distributed single-cell 3′ mRNA profiling

Cellular barcoding of 3′ mRNAs enabled massively parallel profiling of single-cell gene expression and has been implemented in droplet and microwell based platforms. The latter further adds the value for compatibility with low input samples, optical imaging, scalability, and portability. However, ce...

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Detalles Bibliográficos
Autores principales: Dura, Burak, Choi, Jin-Young, Zhang, Kerou, Damsky, William, Thakral, Durga, Bosenberg, Marcus, Craft, Joe, Fan, Rong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6379653/
https://www.ncbi.nlm.nih.gov/pubmed/30462277
http://dx.doi.org/10.1093/nar/gky1173