Start-Stop Assembly: a functionally scarless DNA assembly system optimized for metabolic engineering

DNA assembly allows individual DNA constructs or libraries to be assembled quickly and reliably. Most methods are either: (i) Modular, easily scalable and suitable for combinatorial assembly, but leave undesirable ‘scar’ sequences; or (ii) bespoke (non-modular), scarless but less suitable for constr...

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Detalles Bibliográficos
Autores principales: Taylor, George M, Mordaka, Paweł M, Heap, John T
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2019
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6379671/
https://www.ncbi.nlm.nih.gov/pubmed/30462270
http://dx.doi.org/10.1093/nar/gky1182

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6379671/
https://www.ncbi.nlm.nih.gov/pubmed/30462270
http://dx.doi.org/10.1093/nar/gky1182

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