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Start-Stop Assembly: a functionally scarless DNA assembly system optimized for metabolic engineering
DNA assembly allows individual DNA constructs or libraries to be assembled quickly and reliably. Most methods are either: (i) Modular, easily scalable and suitable for combinatorial assembly, but leave undesirable ‘scar’ sequences; or (ii) bespoke (non-modular), scarless but less suitable for constr...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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Oxford University Press
2019
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6379671/ https://www.ncbi.nlm.nih.gov/pubmed/30462270 http://dx.doi.org/10.1093/nar/gky1182 |