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In vivo characterisation of fluorescent proteins in budding yeast

Fluorescent proteins (FPs) are widely used in many organisms, but are commonly characterised in vitro. However, the in vitro properties may poorly reflect in vivo performance. Therefore, we characterised 27 FPs in vivo using Saccharomyces cerevisiae as model organism. We linked the FPs via a T2A pep...

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Detalles Bibliográficos
Autores principales: Botman, Dennis, de Groot, Daan Hugo, Schmidt, Phillipp, Goedhart, Joachim, Teusink, Bas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6381139/
https://www.ncbi.nlm.nih.gov/pubmed/30783202
http://dx.doi.org/10.1038/s41598-019-38913-z